LOCUS       pLV-EF1a-hHIF1a-IRES-Neo       11452 bp    DNA     circular     28-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
            
FEATURES             Location/Qualifiers
     CDS             9373..10233
                     /label=Amp(R)
     promoter        10434..10974
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             7978..8212
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(8787..9242)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    8415..8628
                     /label=SV40 ori
     misc_feature    7299..7895
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     CDS             6495..7289
                     /label=Neo
     RBS             5899..6483
                     /label=IRES
     CDS             3369..5849
                     /label=hHIF1a
     intron          2361..3303
                     /label=intron
     misc_feature    1807..1929
                     /label=cPPT
     polyA_signal    8284..8414
                     /label=SV40 pA
BASE COUNT     2972 a      2752 c      2889 g      2839 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat ggagggcgcc ggcggcgcga acgacaagaa aaagataagt tctgaacgtc 
     3421 gaaaagaaaa gtctcgagat gcagccagat ctcggcgaag taaagaatct gaagtttttt 
     3481 atgagcttgc tcatcagttg ccacttccac ataatgtgag ttcgcatctt gataaggcct 
     3541 ctgtgatgag gcttaccatc agctatttgc gtgtgaggaa acttctggat gctggtgatt 
     3601 tggatattga agatgacatg aaagcacaga tgaattgctt ttatttgaaa gccttggatg 
     3661 gttttgttat ggttctcaca gatgatggtg acatgattta catttctgat aatgtgaaca 
     3721 aatacatggg attaactcag tttgaactaa ctggacacag tgtgtttgat tttactcatc 
     3781 catgtgacca tgaggaaatg agagaaatgc ttacacacag aaatggcctt gtgaaaaagg 
     3841 gtaaagaaca aaacacacag cgaagctttt ttctcagaat gaagtgtacc ctaactagcc 
     3901 gaggaagaac tatgaacata aagtctgcaa catggaaggt attgcactgc acaggccaca 
     3961 ttcacgtata tgataccaac agtaaccaac ctcagtgtgg gtataagaaa ccacctatga 
     4021 cctgcttggt gctgatttgt gaacccattc ctcacccatc aaatattgaa attcctttag 
     4081 atagcaagac tttcctcagt cgacacagcc tggatatgaa attttcttat tgtgatgaaa 
     4141 gaattaccga attgatggga tatgagccag aagaactttt aggccgctca atttatgaat 
     4201 attatcatgc tttggactct gatcatctga ccaaaactca tcatgatatg tttactaaag 
     4261 gacaagtcac cacaggacag tacaggatgc ttgccaaaag aggtggatat gtctgggttg 
     4321 aaactcaagc aactgtcata tataacacca agaattctca accacagtgc attgtatgtg 
     4381 tgaattacgt tgtgagtggt attattcagc acgacttgat tttctccctt caacaaacag 
     4441 aatgtgtcct taaaccggtt gaatcttcag atatgaaaat gactcagcta ttcaccaaag 
     4501 ttgaatcaga agatacaagt agcctctttg acaaacttaa gaaggaacct gatgctttaa 
     4561 ctttgctggc cccagccgct ggagacacaa tcatatcttt agattttggc agcaacgaca 
     4621 cagaaactga tgaccagcaa cttgaggaag taccattata taatgatgta atgctcccct 
     4681 cacccaacga aaaattacag aatataaatt tggcaatgtc tccattaccc accgctgaaa 
     4741 cgccaaagcc acttcgaagt agtgctgacc ctgcactcaa tcaagaagtt gcattaaaat 
     4801 tagaaccaaa tccagagtca ctggaacttt cttttaccat gccccagatt caggatcaga 
     4861 cacctagtcc ttccgatgga agcactagac aaagttcacc tgagcctaat agtcccagtg 
     4921 aatattgttt ttatgtggat agtgatatgg tcaatgaatt caagttggaa ttggtagaaa 
     4981 aactttttgc tgaagacaca gaagcaaaga acccattttc tactcaggac acagatttag 
     5041 acttggagat gttagctccc tatatcccaa tggatgatga cttccagtta cgttccttcg 
     5101 atcagttgtc accattagaa agcagttccg caagccctga aagcgcaagt cctcaaagca 
     5161 cagttacagt attccagcag actcaaatac aagaacctac tgctaatgcc accactacca 
     5221 ctgccaccac tgatgaatta aaaacagtga caaaagaccg tatggaagac attaaaatat 
     5281 tgattgcatc tccatctcct acccacatac ataaagaaac tactagtgcc acatcatcac 
     5341 catatagaga tactcaaagt cggacagcct caccaaacag agcaggaaaa ggagtcatag 
     5401 aacagacaga aaaatctcat ccaagaagcc ctaacgtgtt atctgtcgct ttgagtcaaa 
     5461 gaactacagt tcctgaggaa gaactaaatc caaagatact agctttgcag aatgctcaga 
     5521 gaaagcgaaa aatggaacat gatggttcac tttttcaagc agtaggaatt ggaacattat 
     5581 tacagcagcc agacgatcat gcagctacta catcactttc ttggaaacgt gtaaaaggat 
     5641 gcaaatctag tgaacagaat ggaatggagc aaaagacaat tattttaata ccctctgatt 
     5701 tagcatgtag actgctgggg caatcaatgg atgaaagtgg attaccacag ctgaccagtt 
     5761 atgattgtga agttaatgct cctatacaag gcagcagaaa cctactgcag ggtgaagaat 
     5821 tactcagagc tttggatcaa gttaactgac aattctgcag atatccatca cactggcggc 
     5881 cgctcgagca tgcatctagc ccctctccct cccccccccc taacgttact ggccgaagcc 
     5941 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt 
     6001 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc 
     6061 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc 
     6121 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc 
     6181 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg 
     6241 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct 
     6301 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat 
     6361 ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc 
     6421 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc 
     6481 acactagagc caccatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 
     6541 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 
     6601 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 
     6661 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 
     6721 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 
     6781 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 
     6841 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 
     6901 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 
     6961 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 
     7021 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 
     7081 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 
     7141 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 
     7201 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
     7261 tctatcgcct tcttgacgag ttcttctgag cggccgcgtc gacaatcaac ctctggatta 
     7321 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 
     7381 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 
     7441 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 
     7501 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 
     7561 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 
     7621 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 
     7681 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg 
     7741 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 
     7801 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 
     7861 gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga 
     7921 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 
     7981 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc 
     8041 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 
     8101 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 
     8161 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 
     8221 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 
     8281 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
     8341 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
     8401 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc 
     8461 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 
     8521 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 
     8581 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt 
     8641 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca 
     8701 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 
     8761 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg 
     8821 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 
     8881 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 
     8941 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 
     9001 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 
     9061 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 
     9121 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 
     9181 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat 
     9241 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 
     9301 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 
     9361 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 
     9421 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 
     9481 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 
     9541 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 
     9601 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 
     9661 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 
     9721 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 
     9781 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 
     9841 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 
     9901 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 
     9961 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 
    10021 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 
    10081 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 
    10141 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 
    10201 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 
    10261 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 
    10321 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 
    10381 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 
    10441 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 
    10501 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 
    10561 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 
    10621 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 
    10681 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 
    10741 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 
    10801 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 
    10861 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 
    10921 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 
    10981 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 
    11041 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 
    11101 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 
    11161 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 
    11221 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 
    11281 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 
    11341 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat 
    11401 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gc 
//
