LOCUS       pLV-EF1a-hBmi1-IRES-Bsd        9523 bp    DNA     circular     28-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
            
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7444..8304
                     /label=Amp(R)
     promoter        8505..9045
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6049..6283
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6858..7313)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6355..6485
                     /label=SV40 pA
     misc_feature    6486..6699
                     /label=SV40 ori
     misc_feature    5370..5966
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             4962..5360
                     /label=Bsd
     RBS             4354..4938
                     /label=IRES
     CDS             3369..4349
                     /label=hBmi-1
BASE COUNT     2403 a      2308 c      2452 g      2360 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat gcatcgaaca acgagaatca agatcactga gctaaatccc cacctgatgt 
     3421 gtgtgctttg tggagggtac ttcattgatg ccacaaccat aatagaatgt ctacattcct 
     3481 tctgtaaaac gtgtattgtt cgttacctgg agaccagcaa gtattgtcct atttgtgatg 
     3541 tccaagttca caagaccaga ccactactga atataaggtc agataaaact ctccaagata 
     3601 ttgtatacaa attagttcca gggcttttca aaaatgaaat gaagagaaga agggattttt 
     3661 atgcagctca tccttctgct gatgctgcca atggctctaa tgaagataga ggagaggttg 
     3721 cagatgaaga taagagaatt ataactgatg atgagataat aagcttatcc attgaattct 
     3781 ttgaccagaa cagattggat cggaaagtaa acaaagacaa agagaaatct aaggaggagg 
     3841 tgaatgataa aagatactta cgatgcccag cagcaatgac tgtgatgcac ttaagaaagt 
     3901 ttctcagaag taaaatggac atacctaata ctttccagat tgatgtcatg tatgaggagg 
     3961 aacctttaaa ggattattat acactaatgg atattgccta catttatacc tggagaagga 
     4021 atggtccact tccattgaaa tacagagttc gacctacttg taaaagaatg aagatcagtc 
     4081 accagagaga tggactgaca aatgctggag aactggaaag tgactctggg agtgacaagg 
     4141 ccaacagccc agcaggaggt attccctcca cctcttcttg tttgcctagc cccagtactc 
     4201 cagtgcagtc tcctcatcca cagtttcctc acatttccag tactatgaat ggaaccagca 
     4261 acagccccag cggtaaccac caatcttctt ttgccaatag acctcgaaaa tcatcagtaa 
     4321 atgggtcatc agcaacttct tctggttgag ctagcccctc tccctccccc ccccctaacg 
     4381 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca 
     4441 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga 
     4501 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga 
     4561 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca 
     4621 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag 
     4681 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa 
     4741 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac 
     4801 cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga 
     4861 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac 
     4921 gatgataata tggccacact agagatccac cggtcgccac catggccaag cctttgtctc 
     4981 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg 
     5041 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca 
     5101 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg 
     5161 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct 
     5221 tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca 
     5281 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg 
     5341 gttatgtgtg ggagggctaa gcggccgcgt cgacaatcaa cctctggatt acaaaatttg 
     5401 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 
     5461 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 
     5521 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 
     5581 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 
     5641 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 
     5701 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 
     5761 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 
     5821 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 
     5881 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 
     5941 ctccctttgg gccgcctccc cgcctggaat tcgagctcgg tacctttaag accaatgact 
     6001 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 
     6061 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac 
     6121 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 
     6181 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 
     6241 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 
     6301 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg 
     6361 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 
     6421 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 
     6481 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 
     6541 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 
     6601 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt 
     6661 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg 
     6721 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt 
     6781 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag 
     6841 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 
     6901 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 
     6961 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 
     7021 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 
     7081 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 
     7141 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 
     7201 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 
     7261 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc 
     7321 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 
     7381 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 
     7441 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 
     7501 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 
     7561 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 
     7621 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 
     7681 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 
     7741 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 
     7801 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 
     7861 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 
     7921 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 
     7981 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 
     8041 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 
     8101 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 
     8161 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 
     8221 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 
     8281 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 
     8341 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 
     8401 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 
     8461 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     8521 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     8581 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 
     8641 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     8701 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     8761 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     8821 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     8881 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     8941 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     9001 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     9061 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     9121 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 
     9181 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 
     9241 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 
     9301 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt 
     9361 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt 
     9421 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag 
     9481 cgcgcaatta accctcacta aagggaacaa aagctggagc tgc 
//
