LOCUS       pLV-EF1a-SV40_large_T-IRES-Neo       11053 bp    DNA     circular     23-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             8974..9834
                     /label=Amp(R)
     promoter        10035..10575
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             7579..7813
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(8388..8843)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    7885..8015
                     /label=SV40 pA
     misc_feature    8016..8229
                     /label=SV40 ori
     misc_feature    6900..7496
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     RBS             5500..6084
                     /label=IRES
     CDS             3369..5495
                     /label=SV40 large T antigen
     CDS             6096..6890
                     /label=Neo
BASE COUNT     2845 a      2549 c      2861 g      2798 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag 
     3421 gtcttgaaag gagtgcctgg gggaatattc ctctgatgag aaaggcatat ttaaaaaaat 
     3481 gcaaggagtt tcatcctgat aaaggaggag atgaagaaaa aatgaagaaa atgaatactc 
     3541 tgtacaagaa aatggaagat ggagtaaaat atgctcatca acctgacttt ggaggcttct 
     3601 gggatgcaac tgagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct 
     3661 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg 
     3721 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact 
     3781 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt 
     3841 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa 
     3901 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc 
     3961 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct 
     4021 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta 
     4081 gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag catgatttta 
     4141 atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca gagtatgcaa 
     4201 tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa tttcagtaca 
     4261 gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat aagtaccatg 
     4321 aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa accatatgcc 
     4381 aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa ttaactagag 
     4441 aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata atgtttggtt 
     4501 ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta cactgtttgt 
     4561 tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac aacattccta 
     4621 aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca ttagcagctg 
     4681 ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg gacaggctga 
     4741 actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat gtaaagggca 
     4801 ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg gacaatttaa 
     4861 gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta aataaaagaa 
     4921 ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct aaaacactgc 
     4981 aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag cattgcctgg 
     5041 aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct ttgcttctta 
     5101 tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc agaattgtgg 
     5161 agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa atgaagttta 
     5221 atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat gatgatgaag 
     5281 acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg gaagactcag 
     5341 ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct cagtcctcac 
     5401 agtctgttca tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 
     5461 aacctcccac acctccccct gaacctgaaa cataagctag cccctctccc tccccccccc 
     5521 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat 
     5581 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct 
     5641 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg 
     5701 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc 
     5761 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg 
     5821 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg 
     5881 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga 
     5941 aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt 
     6001 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa 
     6061 aaacacgatg ataatatggc cacactagag ccaccatgat tgaacaagat ggattgcacg 
     6121 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 
     6181 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 
     6241 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt 
     6301 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 
     6361 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 
     6421 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 
     6481 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 
     6541 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 
     6601 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg 
     6661 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 
     6721 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 
     6781 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 
     6841 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcggccgcgt 
     6901 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 
     6961 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 
     7021 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 
     7081 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 
     7141 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 
     7201 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 
     7261 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 
     7321 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 
     7381 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 
     7441 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 
     7501 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 
     7561 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt 
     7621 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 
     7681 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 
     7741 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 
     7801 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 
     7861 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 
     7921 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 
     7981 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 
     8041 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 
     8101 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 
     8161 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta 
     8221 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 
     8281 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 
     8341 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag 
     8401 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 
     8461 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 
     8521 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 
     8581 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 
     8641 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 
     8701 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 
     8761 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 
     8821 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct 
     8881 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 
     8941 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 
     9001 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 
     9061 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 
     9121 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 
     9181 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 
     9241 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 
     9301 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 
     9361 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 
     9421 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 
     9481 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 
     9541 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 
     9601 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 
     9661 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 
     9721 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 
     9781 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 
     9841 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 
     9901 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 
     9961 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 
    10021 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 
    10081 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 
    10141 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 
    10201 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 
    10261 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 
    10321 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 
    10381 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 
    10441 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 
    10501 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 
    10561 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 
    10621 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 
    10681 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 
    10741 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 
    10801 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 
    10861 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 
    10921 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca 
    10981 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa 
    11041 aagctggagc tgc 
//
