LOCUS       pLV-EF1a-SV40_large_T-IRES-GFP       10991 bp    DNA     circular     23-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             8912..9772
                     /label=Amp(R)
     promoter        9973..10513
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             7517..7751
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(8326..8781)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    7823..7953
                     /label=SV40 pA
     misc_feature    7954..8167
                     /label=SV40 ori
     misc_feature    6838..7434
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     RBS             5500..6084
                     /label=IRES
     CDS             3369..5495
                     /label=SV40 large T antigen
     CDS             6108..6827
                     /label=GFP
BASE COUNT     2878 a      2569 c      2818 g      2726 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag 
     3421 gtcttgaaag gagtgcctgg gggaatattc ctctgatgag aaaggcatat ttaaaaaaat 
     3481 gcaaggagtt tcatcctgat aaaggaggag atgaagaaaa aatgaagaaa atgaatactc 
     3541 tgtacaagaa aatggaagat ggagtaaaat atgctcatca acctgacttt ggaggcttct 
     3601 gggatgcaac tgagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct 
     3661 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg 
     3721 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact 
     3781 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt 
     3841 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa 
     3901 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc 
     3961 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct 
     4021 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta 
     4081 gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag catgatttta 
     4141 atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca gagtatgcaa 
     4201 tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa tttcagtaca 
     4261 gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat aagtaccatg 
     4321 aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa accatatgcc 
     4381 aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa ttaactagag 
     4441 aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata atgtttggtt 
     4501 ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta cactgtttgt 
     4561 tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac aacattccta 
     4621 aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca ttagcagctg 
     4681 ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg gacaggctga 
     4741 actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat gtaaagggca 
     4801 ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg gacaatttaa 
     4861 gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta aataaaagaa 
     4921 ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct aaaacactgc 
     4981 aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag cattgcctgg 
     5041 aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct ttgcttctta 
     5101 tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc agaattgtgg 
     5161 agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa atgaagttta 
     5221 atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat gatgatgaag 
     5281 acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg gaagactcag 
     5341 ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct cagtcctcac 
     5401 agtctgttca tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 
     5461 aacctcccac acctccccct gaacctgaaa cataagctag cccctctccc tccccccccc 
     5521 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat 
     5581 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct 
     5641 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg 
     5701 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc 
     5761 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg 
     5821 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg 
     5881 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga 
     5941 aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt 
     6001 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa 
     6061 aaacacgatg ataatatggc cacactagag atccaccggt cgccaccatg gtgagcaagg 
     6121 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 
     6181 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 
     6241 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 
     6301 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 
     6361 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 
     6421 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 
     6481 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 
     6541 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 
     6601 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 
     6661 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 
     6721 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 
     6781 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgtcg 
     6841 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 
     6901 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 
     6961 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 
     7021 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 
     7081 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 
     7141 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 
     7201 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 
     7261 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 
     7321 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 
     7381 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc 
     7441 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 
     7501 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt 
     7561 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 
     7621 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 
     7681 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 
     7741 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 
     7801 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 
     7861 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
     7921 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg 
     7981 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 
     8041 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 
     8101 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg 
     8161 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 
     8221 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 
     8281 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg 
     8341 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 
     8401 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 
     8461 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 
     8521 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 
     8581 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 
     8641 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 
     8701 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 
     8761 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat 
     8821 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 
     8881 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 
     8941 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 
     9001 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 
     9061 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 
     9121 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 
     9181 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 
     9241 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 
     9301 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 
     9361 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 
     9421 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 
     9481 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 
     9541 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 
     9601 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 
     9661 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 
     9721 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 
     9781 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 
     9841 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 
     9901 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 
     9961 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 
    10021 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 
    10081 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 
    10141 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 
    10201 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 
    10261 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 
    10321 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 
    10381 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 
    10441 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 
    10501 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 
    10561 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 
    10621 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 
    10681 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 
    10741 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 
    10801 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 
    10861 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 
    10921 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 
    10981 gctggagctg c 
//
