LOCUS       pLV-EF1a-SV40_T-IRES-Neo       11399 bp    DNA     circular     23-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             9320..10180
                     /label=Amp(R)
     promoter        10381..10921
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             7925..8159
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(8734..9189)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    8231..8361
                     /label=SV40 pA
     misc_feature    8362..8575
                     /label=SV40 ori
     misc_feature    7246..7842
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     RBS             5846..6430
                     /label=IRES
     CDS             join(3369..3614,3961..5841)
                     /label=SV40 large T antigen
     intron          3615..3960
                     /label=SV40 intron 3
     CDS             3369..3893
                     /label=SV40 small T antigen
     CDS             6442..7236
                     /label=Neo
BASE COUNT     2946 a      2597 c      2936 g      2920 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag 
     3421 gtcttgaaag gagtgcctgg gggaatattc ctctgatgag aaaggcatat ttaaaaaaat 
     3481 gcaaggagtt tcatcctgat aaaggaggag atgaagaaaa aatgaagaaa atgaatactc 
     3541 tgtacaagaa aatggaagat ggagtaaaat atgctcatca acctgacttt ggaggcttct 
     3601 gggatgcaac tgaggtattt gcttcttcct taaatcctgg tgttgatgca atgtactgca 
     3661 aacaatggcc tgagtgtgca aagaaaatgt ctgctaactg catatgcttg ctgtgcttac 
     3721 tgaggatgaa gcatgaaaat agaaaattat acaggaaaga tccacttgtg tgggttgatt 
     3781 gctactgctt cgattgcttt agaatgtggt ttggacttga tctttgtgaa ggaaccttac 
     3841 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat 
     3901 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag 
     3961 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac 
     4021 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat 
     4081 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg 
     4141 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac 
     4201 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc 
     4261 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg 
     4321 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt 
     4381 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tccattttct 
     4441 gttattgagg aaagtttgcc aggtgggtta aaggagcatg attttaatcc agaagaagca 
     4501 gaggaaacta aacaagtgtc ctggaagctt gtaacagagt atgcaatgga aacaaaatgt 
     4561 gatgatgtgt tgttattgct tgggatgtac ttggaatttc agtacagttt tgaaatgtgt 
     4621 ttaaaatgta ttaaaaaaga acagcccagc cactataagt accatgaaaa gcattatgca 
     4681 aatgctgcta tatttgctga cagcaaaaac caaaaaacca tatgccaaca ggctgttgat 
     4741 actgttttag ctaaaaagcg ggttgatagc ctacaattaa ctagagaaca aatgttaaca 
     4801 aacagattta atgatctttt ggataggatg gatataatgt ttggttctac aggctctgct 
     4861 gacatagaag aatggatggc tggagttgct tggctacact gtttgttgcc caaaatggat 
     4921 tcagtggtgt atgacttttt aaaatgcatg gtgtacaaca ttcctaaaaa aagatactgg 
     4981 ctgtttaaag gaccaattga tagtggtaaa actacattag cagctgcttt gcttgaatta 
     5041 tgtgggggga aagctttaaa tgttaatttg cccttggaca ggctgaactt tgagctagga 
     5101 gtagctattg accagttttt agtagttttt gaggatgtaa agggcactgg aggggagtcc 
     5161 agagatttgc cttcaggtca gggaattaat aacctggaca atttaaggga ttatttggat 
     5221 ggcagtgtta aggtaaactt agaaaagaaa cacctaaata aaagaactca aatatttccc 
     5281 cctggaatag tcaccatgaa tgagtacagt gtgcctaaaa cactgcaggc cagatttgta 
     5341 aaacaaatag attttaggcc caaagattat ttaaagcatt gcctggaacg cagtgagttt 
     5401 ttgttagaaa agagaataat tcaaagtggc attgctttgc ttcttatgtt aatttggtac 
     5461 agacctgtgg ctgagtttgc tcaaagtatt cagagcagaa ttgtggagtg gaaagagaga 
     5521 ttggacaaag agtttagttt gtcagtgtat caaaaaatga agtttaatgt ggctatggga 
     5581 attggagttt tagattggct aagaaacagt gatgatgatg atgaagacag ccaggaaaat 
     5641 gctgataaaa atgaagatgg tggggagaag aacatggaag actcagggca tgaaacaggc 
     5701 attgattcac agtcccaagg ctcatttcag gcccctcagt cctcacagtc tgttcatgat 
     5761 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct 
     5821 ccccctgaac ctgaaacata agctagcccc tctccctccc ccccccctaa cgttactggc 
     5881 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 
     5941 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 
     6001 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 
     6061 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 
     6121 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 
     6181 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 
     6241 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 
     6301 atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc gaggttaaaa 
     6361 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa 
     6421 tatggccaca ctagagccac catgattgaa caagatggat tgcacgcagg ttctccggcc 
     6481 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 
     6541 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 
     6601 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg 
     6661 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 
     6721 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 
     6781 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 
     6841 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 
     6901 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 
     6961 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 
     7021 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 
     7081 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 
     7141 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 
     7201 atcgccttct atcgccttct tgacgagttc ttctgagcgg ccgcgtcgac aatcaacctc 
     7261 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 
     7321 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 
     7381 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 
     7441 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 
     7501 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 
     7561 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 
     7621 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 
     7681 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 
     7741 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 
     7801 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc 
     7861 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 
     7921 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg 
     7981 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 
     8041 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 
     8101 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 
     8161 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 
     8221 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
     8281 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 
     8341 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc 
     8401 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 
     8461 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 
     8521 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg 
     8581 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 
     8641 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 
     8701 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg 
     8761 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 
     8821 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 
     8881 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 
     8941 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 
     9001 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 
     9061 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 
     9121 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc 
     9181 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 
     9241 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 
     9301 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 
     9361 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 
     9421 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 
     9481 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 
     9541 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 
     9601 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 
     9661 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 
     9721 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 
     9781 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 
     9841 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 
     9901 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 
     9961 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 
    10021 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 
    10081 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 
    10141 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 
    10201 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 
    10261 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 
    10321 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 
    10381 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 
    10441 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 
    10501 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 
    10561 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 
    10621 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 
    10681 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 
    10741 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 
    10801 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 
    10861 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 
    10921 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 
    10981 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 
    11041 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 
    11101 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 
    11161 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 
    11221 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 
    11281 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 
    11341 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgc 
//
