LOCUS       pLV-EF1a-MCS-IRES-c-Myc        9471 bp    DNA     circular     25-FEB-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7392..8252
                     /label=Amp(R)
     promoter        8453..8993
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5997..6231
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6806..7261)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6303..6433
                     /label=SV40 pA
     misc_feature    6434..6647
                     /label=SV40 ori
     misc_feature    5318..5914
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     RBS             3401..3985
                     /label=IRES
     CDS             3997..5316
                     /label=c-Myc
     misc_feature    3323..3402
                     /label=MCS
BASE COUNT     2313 a      2433 c      2481 g      2244 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 
     3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 
     3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 
     3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 
     3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 
     3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 
     3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 
     3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 
     3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 
     3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 
     3961 aaaacacgat gataatatgg ccacactagt gccaccatgc ccctcaacgt tagcttcacc 
     4021 aacaggaact atgacctcga ctacgactcg gtgcagccgt atttctactg cgacgaggag 
     4081 gagaacttct accagcagca gcagcagagc gagctgcagc ccccggcgcc cagcgaggat 
     4141 atctggaaga aattcgagct gctgcccacc ccgcccctgt cccctagccg ccgctccggg 
     4201 ctctgctcgc cctcctacgt tgcggtcaca cccttctccc ttcggggaga caacgacggc 
     4261 ggtggcggga gcttctccac ggccgaccag ctggagatgg tgaccgagct gctgggagga 
     4321 gacatggtga accagagttt catctgcgac ccggacgacg agaccttcat caaaaacatc 
     4381 atcatccagg actgtatgtg gagcggcttc tcggccgccg ccaagctcgt ctcagagaag 
     4441 ctggcctcct accaggctgc gcgcaaagac agcggcagcc cgaaccccgc ccgcggccac 
     4501 agcgtctgct ccacctccag cttgtacctg caggatctga gcgccgccgc ctcagagtgc 
     4561 atcgacccct cggtggtctt cccctaccct ctcaacgaca gcagctcgcc caagtcctgc 
     4621 gcctcgcaag actccagcgc cttctctccg tcctcggatt ctctgctctc ctcgacggag 
     4681 tcctccccgc agggcagccc cgagcccctg gtgctccatg aggagacacc gcccaccacc 
     4741 agcagcgact ctgaggagga acaagaagat gaggaagaaa tcgatgttgt ttctgtggaa 
     4801 aagaggcagg ctcctggcaa aaggtcagag tctggatcac cttctgctgg aggccacagc 
     4861 aaacctcctc acagcccact ggtcctcaag aggtgccacg tctccacaca tcagcacaac 
     4921 tacgcagcgc ctccctccac tcggaaggac tatcctgctg ccaagagggt caagttggac 
     4981 agtgtcagag tcctgagaca gatcagcaac aaccgaaaat gcaccagccc caggtcctcg 
     5041 gacaccgagg agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca gaggaggaac 
     5101 gagctaaaac ggagcttttt tgccctgcgt gaccagatcc cggagttgga aaacaatgaa 
     5161 aaggccccca aggtagttat ccttaaaaaa gccacagcat acatcctgtc cgtccaagca 
     5221 gaggagcaaa agctcatttc tgaagaggac ttgttgcgga aacgacgaga acagttgaaa 
     5281 cacaaacttg aacagctacg gaactcttgt gcgtaagtcg acaatcaacc tctggattac 
     5341 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 
     5401 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 
     5461 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 
     5521 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 
     5581 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 
     5641 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 
     5701 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg 
     5761 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 
     5821 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg 
     5881 agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta cctttaagac 
     5941 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 
     6001 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct 
     6061 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 
     6121 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 
     6181 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 
     6241 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 
     6301 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 
     6361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 
     6421 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc 
     6481 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 
     6541 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 
     6601 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt 
     6661 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat 
     6721 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag 
     6781 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt 
     6841 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 
     6901 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 
     6961 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 
     7021 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 
     7081 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 
     7141 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 
     7201 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt 
     7261 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 
     7321 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 
     7381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 
     7441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 
     7501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 
     7561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 
     7621 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 
     7681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 
     7741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 
     7801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 
     7861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 
     7921 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 
     7981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 
     8041 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 
     8101 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 
     8161 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 
     8221 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 
     8281 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 
     8341 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
     8401 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
     8461 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
     8521 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 
     8581 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
     8641 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
     8701 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
     8761 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
     8821 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
     8881 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
     8941 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
     9001 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
     9061 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 
     9121 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 
     9181 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 
     9241 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 
     9301 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 
     9361 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 
     9421 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg c 
//
