LOCUS       pLV-EF1a-MCS-IRES-RasV12        8721 bp    DNA     circular     25-FEB-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     CDS             6642..7502
                     /label=Amp(R)
     promoter        7703..8243
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5247..5481
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6056..6511)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5553..5683
                     /label=SV40 pA
     misc_feature    5684..5897
                     /label=SV40 ori
     misc_feature    4568..5164
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     RBS             3401..3985
                     /label=IRES
     CDS             3997..4566
                     /label=RasV12
     misc_feature    3323..3402
                     /label=MCS
     misc_feature    1807..1929
                     /label=cPPT
BASE COUNT     2120 a      2152 c      2322 g      2127 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc ctcccccccc 
     3421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 
     3481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 
     3541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 
     3601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 
     3661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 
     3721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 
     3781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 
     3841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt 
     3901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 
     3961 aaaacacgat gataatatgg ccacactagt gccaccatga cggaatataa gctggtggtg 
     4021 gtgggcgccg tcggtgtggg caagagtgcg ctgaccatcc agctgatcca gaaccatttt 
     4081 gtggacgaat acgaccccac tatagaggat tcctaccgga agcaggtggt cattgatggg 
     4141 gagacgtgcc tgttggacat cctggatacc gccggccagg aggagtacag cgccatgcgg 
     4201 gaccagtaca tgcgcaccgg ggagggcttc ctgtgtgtgt ttgccatcaa caacaccaag 
     4261 tcttttgagg acatccacca gtacagggag cagatcaaac gggtgaagga ctcggatgac 
     4321 gtgcccatgg tgctggtggg gaacaagtgt gacctggctg cacgcactgt ggaatctcgg 
     4381 caggctcagg acctcgcccg aagctacggc atcccctaca tcgagacctc ggccaagacc 
     4441 cggcagggag tggaggatgc cttctacacg ttggtgcgtg agatccggca gcacaagctg 
     4501 cggaagctga accctcctga tgagagtggc cccggctgca tgagctgcaa gtgtgtgctc 
     4561 tcctgagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 
     4621 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 
     4681 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 
     4741 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 
     4801 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 
     4861 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 
     4921 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 
     4981 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 
     5041 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 
     5101 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 
     5161 cctggaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta 
     5221 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 
     5281 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 
     5341 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 
     5401 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 
     5461 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 
     5521 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt 
     5581 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     5641 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc 
     5701 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
     5761 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 
     5821 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag 
     5881 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 
     5941 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 
     6001 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg 
     6061 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 
     6121 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 
     6181 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 
     6241 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 
     6301 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 
     6361 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 
     6421 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 
     6481 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg 
     6541 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 
     6601 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 
     6661 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 
     6721 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 
     6781 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 
     6841 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 
     6901 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 
     6961 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 
     7021 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 
     7081 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 
     7141 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 
     7201 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 
     7261 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 
     7321 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 
     7381 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 
     7441 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 
     7501 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 
     7561 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 
     7621 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 
     7681 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 
     7741 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 
     7801 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 
     7861 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 
     7921 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 
     7981 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 
     8041 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 
     8101 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 
     8161 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 
     8221 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 
     8281 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 
     8341 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 
     8401 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac 
     8461 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 
     8521 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 
     8581 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat 
     8641 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa 
     8701 gggaacaaaa gctggagctg c 
//
