LOCUS       pLV-EF1a-GFP-rLC3-IRES-Puro        9910 bp    DNA     circular     28-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
            
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7831..8691
                     /label=Amp(R)
     promoter        8892..9432
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6436..6670
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7245..7700)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6742..6872
                     /label=SV40 pA
     misc_feature    6873..7086
                     /label=SV40 ori
     misc_feature    5757..6353
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             5148..5747
                     /label=Puro
     RBS             4540..5124
                     /label=IRES
     CDS             3369..4085
                     /label=GFP
     CDS             4104..4529
                     /label=rat LC3
BASE COUNT     2375 a      2596 c      2660 g      2279 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 
     3421 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 
     3481 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 
     3541 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc 
     3601 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 
     3661 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 
     3721 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 
     3781 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc 
     3841 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 
     3901 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 
     3961 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 
     4021 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 
     4081 acaagtccgg actcagatct agaccgtccg agaagacctt caaacagcgc cggagcttcg 
     4141 aacaaagagt ggaagatgtc cggctcatcc gggagcagca ccccaccaag atcccagtga 
     4201 ttatagagcg atacaagggt gagaagcagc tgcccgtcct ggacaagacc aagttccttg 
     4261 tacctgatca cgtgaatatg agcgaactca tcaagataat tagaaggcgc ctgcagctca 
     4321 atgctaacca agccttcttc ctcctggtga atgggcacag catggtgagt gtgtccacac 
     4381 ccatctctga agtgtacgag agcgagagag atgaagacgg cttcctgtac atggtctatg 
     4441 cctcccagga gacgttcggg acagcactgg ctgttacata catgtcagct ctgaaggcaa 
     4501 cagcaacagg aagagagcca tgcttgtgag aattcgctag cccctctccc tccccccccc 
     4561 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat 
     4621 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct 
     4681 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg 
     4741 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc 
     4801 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg 
     4861 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg 
     4921 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga 
     4981 aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt 
     5041 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa 
     5101 aaacacgatg ataatatggc cacactagag atccaccggt cgccaccatg accgagtaca 
     5161 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg 
     5221 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc 
     5281 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt 
     5341 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg 
     5401 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg 
     5461 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc 
     5521 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc 
     5581 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc 
     5641 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc 
     5701 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagcg gccgcgtcga 
     5761 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 
     5821 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 
     5881 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 
     5941 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 
     6001 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 
     6061 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 
     6121 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 
     6181 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 
     6241 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 
     6301 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg 
     6361 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 
     6421 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt 
     6481 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 
     6541 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 
     6601 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 
     6661 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa 
     6721 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc 
     6781 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 
     6841 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc 
     6901 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     6961 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 
     7021 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc 
     7081 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 
     7141 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 
     7201 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg 
     7261 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 
     7321 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 
     7381 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 
     7441 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 
     7501 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 
     7561 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 
     7621 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 
     7681 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt 
     7741 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 
     7801 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 
     7861 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 
     7921 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 
     7981 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 
     8041 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 
     8101 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 
     8161 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 
     8221 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 
     8281 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 
     8341 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 
     8401 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 
     8461 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 
     8521 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 
     8581 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 
     8641 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 
     8701 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 
     8761 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 
     8821 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 
     8881 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 
     8941 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 
     9001 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 
     9061 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 
     9121 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 
     9181 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 
     9241 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 
     9301 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 
     9361 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 
     9421 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 
     9481 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 
     9541 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 
     9601 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 
     9661 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 
     9721 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac 
     9781 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga 
     9841 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag 
     9901 ctggagctgc 
//
