LOCUS       pLV-EF1a-GFP-hLC3b-IRES-Puro        9853 bp    DNA     circular     28-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
            
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7774..8634
                     /label=Amp(R)
     promoter        8835..9375
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6379..6613
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7188..7643)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6685..6815
                     /label=SV40 pA
     misc_feature    6816..7029
                     /label=SV40 ori
     misc_feature    5700..6296
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             5091..5690
                     /label=Puro
     RBS             4483..5067
                     /label=IRES
     CDS             3369..4085
                     /label=GFP
     CDS             4104..4478
                     /label=hLC3b
BASE COUNT     2368 a      2569 c      2637 g      2279 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 
     3421 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 
     3481 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 
     3541 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc 
     3601 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 
     3661 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 
     3721 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 
     3781 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc 
     3841 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 
     3901 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 
     3961 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 
     4021 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 
     4081 acaagtccgg actcagatct agaccgtcgg agaagacctt caagcagcgc cgcaccttcg 
     4141 aacaaagagt agaagatgtc cgacttattc gagagcagca tccaaccaaa atcccggtga 
     4201 taatagaacg atacaagggt gagaagcagc ttcctgttct ggataaaaca aagttccttg 
     4261 tacctgacca tgtcaacatg agtgagctca tcaagataat tagaaggcgc ttacagctca 
     4321 atgctaatca ggccttcttc ctgttggtga acggacacag catggtcagc gtctccacac 
     4381 caatctcaga ggtgtatgag agtgagaaag atgaagatgg attcctgtac atggtctatg 
     4441 cctcccagga gacgttcggg atgaaattgt cagtgtaagc tagcccctct ccctcccccc 
     4501 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt 
     4561 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct 
     4621 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga 
     4681 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga 
     4741 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac 
     4801 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag 
     4861 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc 
     4921 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg 
     4981 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt 
     5041 gaaaaacacg atgataatat ggccacacta gagatccacc ggtcgccacc atgaccgagt 
     5101 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg 
     5161 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg 
     5221 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 
     5281 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 
     5341 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 
     5401 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 
     5461 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 
     5521 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 
     5581 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 
     5641 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gcggccgcgt 
     5701 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 
     5761 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 
     5821 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 
     5881 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 
     5941 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 
     6001 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 
     6061 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 
     6121 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 
     6181 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 
     6241 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 
     6301 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 
     6361 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt 
     6421 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 
     6481 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 
     6541 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 
     6601 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 
     6661 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 
     6721 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 
     6781 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 
     6841 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 
     6901 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 
     6961 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta 
     7021 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 
     7081 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 
     7141 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag 
     7201 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 
     7261 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 
     7321 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 
     7381 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 
     7441 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 
     7501 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 
     7561 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 
     7621 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct 
     7681 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 
     7741 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 
     7801 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 
     7861 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 
     7921 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 
     7981 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 
     8041 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 
     8101 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 
     8161 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 
     8221 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 
     8281 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 
     8341 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 
     8401 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 
     8461 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 
     8521 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 
     8581 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 
     8641 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 
     8701 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 
     8761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 
     8821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 
     8881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 
     8941 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 
     9001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 
     9061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 
     9121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 
     9181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 
     9241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 
     9301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 
     9361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 
     9421 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 
     9481 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 
     9541 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 
     9601 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 
     9661 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 
     9721 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca 
     9781 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa 
     9841 aagctggagc tgc 
//
