LOCUS       pLV-EF1a-GFP-Bsd-IRES-c-Myc   10577 bp    DNA     circular     25-FEB-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     CDS             8498..9358
                     /label=Amp(R)
     promoter        9559..10099
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             7103..7337
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7912..8367)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    7540..7753
                     /label=SV40 ori
     misc_feature    6424..7020
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     RBS             4507..5091
                     /label=IRES
     CDS             5103..6422
                     /label=c-Myc
     CDS             3369..4502
                     /label=GFP-Bsd
     misc_feature    1807..1929
                     /label=cPPT
     insertion_seq   2361..3303
                     /label=intron
     polyA_signal    7409..7539
                     /label=SV40 pA
BASE COUNT     2570 a      2782 c      2793 g      2432 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 
     3421 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 
     3481 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 
     3541 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc 
     3601 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 
     3661 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 
     3721 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 
     3781 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc 
     3841 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 
     3901 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 
     3961 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 
     4021 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 
     4081 acaaggaccc accggtcgcc accatggcca agcctttgtc tcaagaagaa tccaccctca 
     4141 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca 
     4201 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg 
     4261 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc 
     4321 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt 
     4381 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg 
     4441 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct 
     4501 aagctagccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag 
     4561 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga 
     4621 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg 
     4681 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt 
     4741 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca 
     4801 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc 
     4861 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat 
     4921 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc 
     4981 ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga 
     5041 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac actagtgcca 
     5101 ccatgcccct caacgttagc ttcaccaaca ggaactatga cctcgactac gactcggtgc 
     5161 agccgtattt ctactgcgac gaggaggaga acttctacca gcagcagcag cagagcgagc 
     5221 tgcagccccc ggcgcccagc gaggatatct ggaagaaatt cgagctgctg cccaccccgc 
     5281 ccctgtcccc tagccgccgc tccgggctct gctcgccctc ctacgttgcg gtcacaccct 
     5341 tctcccttcg gggagacaac gacggcggtg gcgggagctt ctccacggcc gaccagctgg 
     5401 agatggtgac cgagctgctg ggaggagaca tggtgaacca gagtttcatc tgcgacccgg 
     5461 acgacgagac cttcatcaaa aacatcatca tccaggactg tatgtggagc ggcttctcgg 
     5521 ccgccgccaa gctcgtctca gagaagctgg cctcctacca ggctgcgcgc aaagacagcg 
     5581 gcagcccgaa ccccgcccgc ggccacagcg tctgctccac ctccagcttg tacctgcagg 
     5641 atctgagcgc cgccgcctca gagtgcatcg acccctcggt ggtcttcccc taccctctca 
     5701 acgacagcag ctcgcccaag tcctgcgcct cgcaagactc cagcgccttc tctccgtcct 
     5761 cggattctct gctctcctcg acggagtcct ccccgcaggg cagccccgag cccctggtgc 
     5821 tccatgagga gacaccgccc accaccagca gcgactctga ggaggaacaa gaagatgagg 
     5881 aagaaatcga tgttgtttct gtggaaaaga ggcaggctcc tggcaaaagg tcagagtctg 
     5941 gatcaccttc tgctggaggc cacagcaaac ctcctcacag cccactggtc ctcaagaggt 
     6001 gccacgtctc cacacatcag cacaactacg cagcgcctcc ctccactcgg aaggactatc 
     6061 ctgctgccaa gagggtcaag ttggacagtg tcagagtcct gagacagatc agcaacaacc 
     6121 gaaaatgcac cagccccagg tcctcggaca ccgaggagaa tgtcaagagg cgaacacaca 
     6181 acgtcttgga gcgccagagg aggaacgagc taaaacggag cttttttgcc ctgcgtgacc 
     6241 agatcccgga gttggaaaac aatgaaaagg cccccaaggt agttatcctt aaaaaagcca 
     6301 cagcatacat cctgtccgtc caagcagagg agcaaaagct catttctgaa gaggacttgt 
     6361 tgcggaaacg acgagaacag ttgaaacaca aacttgaaca gctacggaac tcttgtgcgt 
     6421 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 
     6481 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 
     6541 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 
     6601 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 
     6661 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 
     6721 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 
     6781 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat 
     6841 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 
     6901 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 
     6961 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 
     7021 gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 
     7081 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct 
     7141 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 
     7201 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 
     7261 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 
     7321 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 
     7381 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 
     7441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     7501 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta 
     7561 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 
     7621 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 
     7681 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt 
     7741 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 
     7801 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 
     7861 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct 
     7921 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 
     7981 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 
     8041 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 
     8101 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 
     8161 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 
     8221 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 
     8281 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 
     8341 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac 
     8401 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 
     8461 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 
     8521 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 
     8581 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 
     8641 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 
     8701 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 
     8761 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 
     8821 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 
     8881 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 
     8941 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 
     9001 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 
     9061 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 
     9121 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 
     9181 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 
     9241 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 
     9301 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 
     9361 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 
     9421 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 
     9481 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 
     9541 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 
     9601 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 
     9661 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 
     9721 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 
     9781 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 
     9841 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 
     9901 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 
     9961 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 
    10021 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 
    10081 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 
    10141 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 
    10201 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 
    10261 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 
    10321 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 
    10381 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 
    10441 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 
    10501 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga 
    10561 acaaaagctg gagctgc 
//
