LOCUS       pLV-EF1a-GFP-Bsd-IRES-RasV12        9827 bp    DNA     circular     25-FEB-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
            
FEATURES             Location/Qualifiers
     CDS             7748..8608
                     /label=Amp(R)
     promoter        8809..9349
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6353..6587
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7162..7617)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    6790..7003
                     /label=SV40 ori
     misc_feature    5674..6270
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     RBS             4507..5091
                     /label=IRES
     CDS             5103..5672
                     /label=RasV12
     CDS             3369..4502
                     /label=GFP-Bsd
     misc_feature    1807..1929
                     /label=cPPT
BASE COUNT     2377 a      2501 c      2634 g      2315 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 
     3421 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 
     3481 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 
     3541 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc 
     3601 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 
     3661 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 
     3721 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 
     3781 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc 
     3841 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 
     3901 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 
     3961 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 
     4021 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 
     4081 acaaggaccc accggtcgcc accatggcca agcctttgtc tcaagaagaa tccaccctca 
     4141 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca 
     4201 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg 
     4261 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc 
     4321 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt 
     4381 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg 
     4441 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct 
     4501 aagctagccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag 
     4561 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga 
     4621 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg 
     4681 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt 
     4741 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca 
     4801 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc 
     4861 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat 
     4921 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc 
     4981 ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga 
     5041 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac actagtgcca 
     5101 ccatgacgga atataagctg gtggtggtgg gcgccgtcgg tgtgggcaag agtgcgctga 
     5161 ccatccagct gatccagaac cattttgtgg acgaatacga ccccactata gaggattcct 
     5221 accggaagca ggtggtcatt gatggggaga cgtgcctgtt ggacatcctg gataccgccg 
     5281 gccaggagga gtacagcgcc atgcgggacc agtacatgcg caccggggag ggcttcctgt 
     5341 gtgtgtttgc catcaacaac accaagtctt ttgaggacat ccaccagtac agggagcaga 
     5401 tcaaacgggt gaaggactcg gatgacgtgc ccatggtgct ggtggggaac aagtgtgacc 
     5461 tggctgcacg cactgtggaa tctcggcagg ctcaggacct cgcccgaagc tacggcatcc 
     5521 cctacatcga gacctcggcc aagacccggc agggagtgga ggatgccttc tacacgttgg 
     5581 tgcgtgagat ccggcagcac aagctgcgga agctgaaccc tcctgatgag agtggccccg 
     5641 gctgcatgag ctgcaagtgt gtgctctcct gagtcgacaa tcaacctctg gattacaaaa 
     5701 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 
     5761 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 
     5821 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 
     5881 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 
     5941 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 
     6001 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 
     6061 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc 
     6121 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 
     6181 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 
     6241 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat 
     6301 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 
     6361 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt 
     6421 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 
     6481 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 
     6541 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 
     6601 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 
     6661 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 
     6721 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 
     6781 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc 
     6841 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 
     6901 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg 
     6961 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac 
     7021 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc 
     7081 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc 
     7141 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 
     7201 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 
     7261 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 
     7321 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 
     7381 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 
     7441 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 
     7501 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 
     7561 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg 
     7621 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 
     7681 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 
     7741 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 
     7801 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 
     7861 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 
     7921 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 
     7981 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 
     8041 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 
     8101 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 
     8161 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 
     8221 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 
     8281 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 
     8341 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 
     8401 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 
     8461 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 
     8521 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 
     8581 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 
     8641 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 
     8701 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 
     8761 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 
     8821 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 
     8881 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 
     8941 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 
     9001 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 
     9061 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 
     9121 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 
     9181 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 
     9241 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 
     9301 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 
     9361 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 
     9421 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 
     9481 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 
     9541 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 
     9601 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 
     9661 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 
     9721 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc 
     9781 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgc 
//
