LOCUS pLV-EF1a-hc-Myc-IRES-Bsd 9861 bp DNA circular 12-MAR-2008 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7782..8642 /label=Amp(R) promoter 8843..9383 /label=pUC ori promoter 7..235 /label=RSV LTR 6387..6621 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(7196..7651) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 6824..7037 /label=SV40 ori promoter 1933..3322 /label=human EF1a promoter CDS 5300..5698 /label=Bsd RBS 4692..5276 /label=IRES CDS 3368..4687 /label=human c-Myc BASE COUNT 2398 a 2541 c 2592 g 2330 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cggttggatc 3361 cgccaccatg cccctcaacg ttagcttcac caacaggaac tatgacctcg actacgactc 3421 ggtgcagccg tatttctact gcgacgagga ggagaacttc taccagcagc agcagcagag 3481 cgagctgcag cccccggcgc ccagcgagga tatctggaag aaattcgagc tgctgcccac 3541 cccgcccctg tcccctagcc gccgctccgg gctctgctcg ccctcctacg ttgcggtcac 3601 acccttctcc cttcggggag acaacgacgg cggtggcggg agcttctcca cggccgacca 3661 gctggagatg gtgaccgagc tgctgggagg agacatggtg aaccagagtt tcatctgcga 3721 cccggacgac gagaccttca tcaaaaacat catcatccag gactgtatgt ggagcggctt 3781 ctcggccgcc gccaagctcg tctcagagaa gctggcctcc taccaggctg cgcgcaaaga 3841 cagcggcagc ccgaaccccg cccgcggcca cagcgtctgc tccacctcca gcttgtacct 3901 gcaggatctg agcgccgccg cctcagagtg catcgacccc tcggtggtct tcccctaccc 3961 tctcaacgac agcagctcgc ccaagtcctg cgcctcgcaa gactccagcg ccttctctcc 4021 gtcctcggat tctctgctct cctcgacgga gtcctccccg cagggcagcc ccgagcccct 4081 ggtgctccat gaggagacac cgcccaccac cagcagcgac tctgaggagg aacaagaaga 4141 tgaggaagaa atcgatgttg tttctgtgga aaagaggcag gctcctggca aaaggtcaga 4201 gtctggatca ccttctgctg gaggccacag caaacctcct cacagcccac tggtcctcaa 4261 gaggtgccac gtctccacac atcagcacaa ctacgcagcg cctccctcca ctcggaagga 4321 ctatcctgct gccaagaggg tcaagttgga cagtgtcaga gtcctgagac agatcagcaa 4381 caaccgaaaa tgcaccagcc ccaggtcctc ggacaccgag gagaatgtca agaggcgaac 4441 acacaacgtc ttggagcgcc agaggaggaa cgagctaaaa cggagctttt ttgccctgcg 4501 tgaccagatc ccggagttgg aaaacaatga aaaggccccc aaggtagtta tccttaaaaa 4561 agccacagca tacatcctgt ccgtccaagc agaggagcaa aagctcattt ctgaagagga 4621 cttgttgcgg aaacgacgag aacagttgaa acacaaactt gaacagctac ggaactcttg 4681 tgcgtaagct agcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga 4741 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa 4801 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc 4861 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc 4921 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg 4981 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca 5041 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag 5101 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct 5161 ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc 5221 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacactag 5281 agatccaccg gtcgccacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga 5341 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc 5401 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg 5461 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac 5521 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg 5581 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca 5641 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagc 5701 ggccgcgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 5761 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 5821 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 5881 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 5941 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 6001 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 6061 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 6121 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 6181 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 6241 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 6301 cctggaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta 6361 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 6421 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 6481 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 6541 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 6601 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 6661 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt 6721 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 6781 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc 6841 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 6901 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 6961 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag 7021 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 7081 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 7141 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg 7201 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 7261 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 7321 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 7381 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 7441 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 7501 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 7561 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 7621 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg 7681 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 7741 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 7801 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 7861 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 7921 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 7981 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 8041 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 8101 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 8161 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 8221 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 8281 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 8341 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 8401 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 8461 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 8521 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 8581 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 8641 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 8701 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 8761 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 8821 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 8881 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 8941 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 9001 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 9061 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 9121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 9181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 9241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 9301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 9361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 9421 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 9481 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 9541 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac 9601 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 9661 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 9721 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat 9781 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa 9841 gggaacaaaa gctggagctg c //