LOCUS pLV-EF1a-hKlf4-IRES-Puro 10156 bp DNA circular 1-MAY-2008 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 8077..8937 /label=Amp(R) promoter 9138..9678 /label=pUC ori promoter 7..235 /label=RSV LTR 6682..6916 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(7491..7946) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 7119..7332 /label=SV40 ori promoter 1933..3322 /label=human EF1a promoter CDS 5394..5993 /label=Puro RBS 4786..5370 /label=IRES CDS 3369..4781 /label=human Klf4 BASE COUNT 2339 a 2749 c 2753 g 2315 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccgccaccat ggctgtcagc gacgcgctgc tcccatcttt ctccacgttc gcgtctggcc 3421 cggcgggaag ggagaagaca ctgcgtcaag caggtgcccc gaataaccgc tggcgggagg 3481 agctctccca catgaagcga cttcccccag tgcttcccgg ccgcccctat gacctggcgg 3541 cggcgaccgt ggccacagac ctggagagcg gcggagccgg tgcggcttgc ggcggtagca 3601 acctggcgcc cctacctcgg agagagaccg aggagttcaa cgatctcctg gacctggact 3661 ttattctctc caattcgctg acccatcctc cggagtcagt ggccgccacc gtgtcctcgt 3721 cagcgtcagc ctcctcttcg tcgtcgccgt cgagcagcgg ccctgccagc gcgccctcca 3781 cctgcagctt cacctatccg atccgggccg ggaacgaccc gggcgtggcg ccgggcggca 3841 cgggcggagg cctcctctat ggcagggagt ccgctccccc tccgacggct cccttcaacc 3901 tggcggacat caacgacgtg agcccctcgg gcggcttcgt ggccgagctc ctgcggccag 3961 aattggaccc ggtgtacatt ccgccgcagc agccgcagcc gccaggtggc gggctgatgg 4021 gcaagttcgt gctgaaggcg tcgctgagcg cccctggcag cgagtacggc agcccgtcgg 4081 tcatcagcgt cagcaaaggc agccctgacg gcagccaccc ggtggtggtg gcgccctaca 4141 acggcgggcc gccgcgcacg tgccccaaga tcaagcagga ggcggtctct tcgtgcaccc 4201 acttgggcgc tggaccccct ctcagcaatg gccaccggcc ggctgcacac gacttccccc 4261 tggggcggca gctccccagc aggactaccc cgaccctggg tcttgaggaa gtgctgagca 4321 gcagggactg tcaccctgcc ctgccgcttc ctcccggctt ccatccccac ccggggccca 4381 attacccatc cttcctgccc gatcagatgc agccgcaagt cccgccgctc cattaccaag 4441 agctcatgcc acccggttcc tgcatgccag aggagcccaa gccaaagagg ggaagacgat 4501 cgtggccccg gaaaaggacc gccacccaca cttgtgatta cgcgggctgc ggcaaaacct 4561 acacaaagag ttcccatctc aaggcacacc tgcgaaccca cacaggtgag aaaccttacc 4621 actgtgactg ggacggctgt ggatggaaat tcgcccgctc agatgaactg accaggcact 4681 accgtaaaca cacggggcac cgcccgttcc agtgccaaaa atgcgaccga gcattttcca 4741 ggtcggacca cctcgcctta cacatgaaga ggcattttta agctagcccc tctccctccc 4801 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata 4861 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg 4921 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt 4981 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag 5041 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc 5101 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga 5161 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg 5221 cccagaaggt accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat 5281 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc 5341 tttgaaaaac acgatgataa tatggccaca ctagagatcc accggtcgcc accatgaccg 5401 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc 5461 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca 5521 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 5581 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 5641 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 5701 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 5761 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 5821 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 5881 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 5941 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagcggccg 6001 cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 6061 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 6121 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 6181 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 6241 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 6301 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 6361 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 6421 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 6481 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 6541 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 6601 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 6661 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg 6721 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 6781 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 6841 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 6901 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 6961 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 7021 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 7081 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa 7141 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 7201 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 7261 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta 7321 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 7381 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 7441 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg 7501 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 7561 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 7621 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 7681 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 7741 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 7801 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 7861 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 7921 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc 7981 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 8041 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 8101 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 8161 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 8221 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 8281 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 8341 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 8401 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 8461 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 8521 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 8581 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 8641 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 8701 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 8761 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 8821 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 8881 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 8941 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 9001 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 9061 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 9121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 9181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 9241 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 9301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 9361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 9421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 9481 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 9541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 9601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 9661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 9721 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 9781 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 9841 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 9901 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 9961 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 10021 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 10081 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa 10141 caaaagctgg agctgc //