LOCUS pLV-EF1a-hKlf4-IRES-Neo 10339 bp DNA circular 1-MAY-2008 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 8260..9120 /label=Amp(R) promoter 9321..9861 /label=pUC ori promoter 7..235 /label=RSV LTR 6865..7099 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(7674..8129) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 7302..7515 /label=SV40 ori promoter 1933..3322 /label=human EF1a promoter CDS 5382..6176 /label=Neo RBS 4786..5370 /label=IRES CDS 3369..4781 /label=human Klf4 BASE COUNT 2394 a 2735 c 2795 g 2415 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccgccaccat ggctgtcagc gacgcgctgc tcccatcttt ctccacgttc gcgtctggcc 3421 cggcgggaag ggagaagaca ctgcgtcaag caggtgcccc gaataaccgc tggcgggagg 3481 agctctccca catgaagcga cttcccccag tgcttcccgg ccgcccctat gacctggcgg 3541 cggcgaccgt ggccacagac ctggagagcg gcggagccgg tgcggcttgc ggcggtagca 3601 acctggcgcc cctacctcgg agagagaccg aggagttcaa cgatctcctg gacctggact 3661 ttattctctc caattcgctg acccatcctc cggagtcagt ggccgccacc gtgtcctcgt 3721 cagcgtcagc ctcctcttcg tcgtcgccgt cgagcagcgg ccctgccagc gcgccctcca 3781 cctgcagctt cacctatccg atccgggccg ggaacgaccc gggcgtggcg ccgggcggca 3841 cgggcggagg cctcctctat ggcagggagt ccgctccccc tccgacggct cccttcaacc 3901 tggcggacat caacgacgtg agcccctcgg gcggcttcgt ggccgagctc ctgcggccag 3961 aattggaccc ggtgtacatt ccgccgcagc agccgcagcc gccaggtggc gggctgatgg 4021 gcaagttcgt gctgaaggcg tcgctgagcg cccctggcag cgagtacggc agcccgtcgg 4081 tcatcagcgt cagcaaaggc agccctgacg gcagccaccc ggtggtggtg gcgccctaca 4141 acggcgggcc gccgcgcacg tgccccaaga tcaagcagga ggcggtctct tcgtgcaccc 4201 acttgggcgc tggaccccct ctcagcaatg gccaccggcc ggctgcacac gacttccccc 4261 tggggcggca gctccccagc aggactaccc cgaccctggg tcttgaggaa gtgctgagca 4321 gcagggactg tcaccctgcc ctgccgcttc ctcccggctt ccatccccac ccggggccca 4381 attacccatc cttcctgccc gatcagatgc agccgcaagt cccgccgctc cattaccaag 4441 agctcatgcc acccggttcc tgcatgccag aggagcccaa gccaaagagg ggaagacgat 4501 cgtggccccg gaaaaggacc gccacccaca cttgtgatta cgcgggctgc ggcaaaacct 4561 acacaaagag ttcccatctc aaggcacacc tgcgaaccca cacaggtgag aaaccttacc 4621 actgtgactg ggacggctgt ggatggaaat tcgcccgctc agatgaactg accaggcact 4681 accgtaaaca cacggggcac cgcccgttcc agtgccaaaa atgcgaccga gcattttcca 4741 ggtcggacca cctcgcctta cacatgaaga ggcattttta agctagcccc tctccctccc 4801 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata 4861 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg 4921 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt 4981 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag 5041 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc 5101 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga 5161 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg 5221 cccagaaggt accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat 5281 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc 5341 tttgaaaaac acgatgataa tatggccaca ctagagccac catgattgaa caagatggat 5401 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 5461 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 5521 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc 5581 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 5641 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 5701 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 5761 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 5821 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 5881 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga 5941 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 6001 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 6061 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 6121 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 6181 ccgcgtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa 6241 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat 6301 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta 6361 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc 6421 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt 6481 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg 6541 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc 6601 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc 6661 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct 6721 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc 6781 tggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 6841 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat 6901 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 6961 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 7021 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 7081 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 7141 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 7201 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 7261 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 7321 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 7381 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 7441 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc 7501 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt 7561 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga 7621 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc 7681 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 7741 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 7801 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 7861 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 7921 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 7981 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 8041 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 8101 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga 8161 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 8221 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 8281 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 8341 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 8401 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 8461 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 8521 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 8581 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 8641 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 8701 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 8761 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 8821 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 8881 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 8941 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 9001 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 9061 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 9121 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 9181 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 9241 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 9301 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 9361 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 9421 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 9481 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 9541 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 9601 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 9661 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 9721 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 9781 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 9841 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 9901 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 9961 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 10021 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 10081 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 10141 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag 10201 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt 10261 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg 10321 gaacaaaagc tggagctgc //