LOCUS       pLV-EF1a-hKlf4-IRES-Bsd    9955 bp    DNA     circular     31-MAY-2009
COMMENT     
COMMENT     This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             7876..8736
                     /label=Amp(R)
     promoter        8937..9477
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6481..6715
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7290..7745)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    6918..7131
                     /label=SV40 ori
     promoter        1933..3322
                     /label=human EF1a promoter
     CDS             5394..5792
                     /label=Bsd
     RBS             4786..5370
                     /label=IRES
     CDS             3369..4781
                     /label=human kLF4
BASE COUNT     2341 a      2620 c      2663 g      2331 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat ggctgtcagc gacgcgctgc tcccatcttt ctccacgttc gcgtctggcc 
     3421 cggcgggaag ggagaagaca ctgcgtcaag caggtgcccc gaataaccgc tggcgggagg 
     3481 agctctccca catgaagcga cttcccccag tgcttcccgg ccgcccctat gacctggcgg 
     3541 cggcgaccgt ggccacagac ctggagagcg gcggagccgg tgcggcttgc ggcggtagca 
     3601 acctggcgcc cctacctcgg agagagaccg aggagttcaa cgatctcctg gacctggact 
     3661 ttattctctc caattcgctg acccatcctc cggagtcagt ggccgccacc gtgtcctcgt 
     3721 cagcgtcagc ctcctcttcg tcgtcgccgt cgagcagcgg ccctgccagc gcgccctcca 
     3781 cctgcagctt cacctatccg atccgggccg ggaacgaccc gggcgtggcg ccgggcggca 
     3841 cgggcggagg cctcctctat ggcagggagt ccgctccccc tccgacggct cccttcaacc 
     3901 tggcggacat caacgacgtg agcccctcgg gcggcttcgt ggccgagctc ctgcggccag 
     3961 aattggaccc ggtgtacatt ccgccgcagc agccgcagcc gccaggtggc gggctgatgg 
     4021 gcaagttcgt gctgaaggcg tcgctgagcg cccctggcag cgagtacggc agcccgtcgg 
     4081 tcatcagcgt cagcaaaggc agccctgacg gcagccaccc ggtggtggtg gcgccctaca 
     4141 acggcgggcc gccgcgcacg tgccccaaga tcaagcagga ggcggtctct tcgtgcaccc 
     4201 acttgggcgc tggaccccct ctcagcaatg gccaccggcc ggctgcacac gacttccccc 
     4261 tggggcggca gctccccagc aggactaccc cgaccctggg tcttgaggaa gtgctgagca 
     4321 gcagggactg tcaccctgcc ctgccgcttc ctcccggctt ccatccccac ccggggccca 
     4381 attacccatc cttcctgccc gatcagatgc agccgcaagt cccgccgctc cattaccaag 
     4441 agctcatgcc acccggttcc tgcatgccag aggagcccaa gccaaagagg ggaagacgat 
     4501 cgtggccccg gaaaaggacc gccacccaca cttgtgatta cgcgggctgc ggcaaaacct 
     4561 acacaaagag ttcccatctc aaggcacacc tgcgaaccca cacaggtgag aaaccttacc 
     4621 actgtgactg ggacggctgt ggatggaaat tcgcccgctc agatgaactg accaggcact 
     4681 accgtaaaca cacggggcac cgcccgttcc agtgccaaaa atgcgaccga gcattttcca 
     4741 ggtcggacca cctcgcctta cacatgaaga ggcattttta agctagcccc tctccctccc 
     4801 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata 
     4861 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg 
     4921 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt 
     4981 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag 
     5041 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc 
     5101 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga 
     5161 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg 
     5221 cccagaaggt accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat 
     5281 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc 
     5341 tttgaaaaac acgatgataa tatggccaca ctagagatcc accggtcgcc accatggcca 
     5401 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca 
     5461 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct 
     5521 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg 
     5581 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga 
     5641 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg 
     5701 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat 
     5761 tgctgccctc tggttatgtg tgggagggct aagcggccgc gtcgacaatc aacctctgga 
     5821 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 
     5881 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 
     5941 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 
     6001 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 
     6061 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 
     6121 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 
     6181 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 
     6241 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 
     6301 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 
     6361 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta 
     6421 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 
     6481 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct 
     6541 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
     6601 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
     6661 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 
     6721 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 
     6781 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
     6841 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 
     6901 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa 
     6961 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 
     7021 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 
     7081 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt 
     7141 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 
     7201 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 
     7261 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc 
     7321 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 
     7381 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 
     7441 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 
     7501 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 
     7561 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 
     7621 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 
     7681 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 
     7741 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 
     7801 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 
     7861 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 
     7921 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 
     7981 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 
     8041 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 
     8101 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 
     8161 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 
     8221 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 
     8281 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 
     8341 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 
     8401 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 
     8461 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 
     8521 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 
     8581 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 
     8641 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 
     8701 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 
     8761 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 
     8821 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 
     8881 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 
     8941 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 
     9001 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 
     9061 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 
     9121 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 
     9181 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 
     9241 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 
     9301 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 
     9361 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 
     9421 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 
     9481 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 
     9541 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 
     9601 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 
     9661 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 
     9721 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 
     9781 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 
     9841 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat 
     9901 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgc 
//
