LOCUS       pLV-EF1a-hLin28-IRES-Neo    9562 bp    DNA     circular     13-SEP-2009
COMMENT     
COMMENT     This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             7483..8343
                     /label=Amp(R)
     promoter        8544..9084
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6897..7352)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    6525..6738
                     /label=SV40 ori
     promoter        1933..3322
                     /label=human EF1a promoter
     RBS             4009..4593
                     /label=IRES
     CDS             3369..3998
                     /label=human Lin28
     CDS             4605..5399
                     /label=Neo
     LTR             6088..6322
                     /label=3' LTR
BASE COUNT     2283 a      2398 c      2587 g      2294 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat gggctccgtg tccaaccagc agtttgcagg tggctgcgcc aaggcggcag 
     3421 aagaggcgcc cgaggaggcg ccggaggacg cggcccgggc ggcggacgag cctcagctgc 
     3481 tgcacggtgc gggcatctgt aagtggttca acgtgcgcat ggggttcggc ttcctgtcca 
     3541 tgaccgcccg cgccggggtc gcgctcgacc ccccagtgga tgtctttgtg caccagagta 
     3601 agctgcacat ggaagggttc cggagcttga aggagggtga ggcagtggag ttcaccttta 
     3661 agaagtcagc caagggtctg gaatccatcc gtgtcaccgg acctggtgga gtattctgta 
     3721 ttgggagtga gaggcggcca aaaggaaaga gcatgcagaa gcgcagatca aaaggagaca 
     3781 ggtgctacaa ctgtggaggt ctagatcatc atgccaagga atgcaagctg ccaccccagc 
     3841 ccaagaagtg ccacttctgc cagagcatca gccatatggt agcctcatgt ccgctgaagg 
     3901 cccagcaggg ccctagtgca cagggaaagc caacctactt tcgagaggaa gaagaagaaa 
     3961 tccacagccc taccctgctc ccggaggcac agaattgagc cacagctagc ccctctccct 
     4021 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct 
     4081 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc 
     4141 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc 
     4201 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg 
     4261 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa 
     4321 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt 
     4381 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg 
     4441 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta 
     4501 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt 
     4561 tcctttgaaa aacacgatga taatatggcc acactagagc caccatgatt gaacaagatg 
     4621 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 
     4681 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 
     4741 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc 
     4801 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 
     4861 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 
     4921 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 
     4981 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 
     5041 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 
     5101 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg 
     5161 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 
     5221 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 
     5281 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 
     5341 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 
     5401 cggccgcgtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 
     5461 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 
     5521 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 
     5581 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 
     5641 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 
     5701 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 
     5761 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 
     5821 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 
     5881 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 
     5941 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 
     6001 gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 
     6061 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa 
     6121 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 
     6181 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 
     6241 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 
     6301 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 
     6361 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 
     6421 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
     6481 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 
     6541 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 
     6601 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 
     6661 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga 
     6721 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 
     6781 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 
     6841 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc 
     6901 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 
     6961 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 
     7021 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 
     7081 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 
     7141 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 
     7201 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 
     7261 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 
     7321 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc 
     7381 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 
     7441 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 
     7501 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 
     7561 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 
     7621 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 
     7681 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 
     7741 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 
     7801 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 
     7861 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 
     7921 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 
     7981 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 
     8041 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 
     8101 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 
     8161 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 
     8221 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 
     8281 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 
     8341 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 
     8401 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 
     8461 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 
     8521 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 
     8581 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 
     8641 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 
     8701 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 
     8761 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 
     8821 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 
     8881 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 
     8941 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 
     9001 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 
     9061 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 
     9121 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 
     9181 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 
     9241 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 
     9301 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 
     9361 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 
     9421 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 
     9481 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa 
     9541 agggaacaaa agctggagct gc 
//
