LOCUS pLV-EF1a-hLin28-IRES-Bsd 9178 bp DNA circular 12-MAR-2008 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7099..7959 /label=Amp(R) promoter 8160..8700 /label=pUC ori promoter 7..235 /label=RSV LTR 5704..5938 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(6513..6968) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 6141..6354 /label=SV40 ori promoter 1933..3322 /label=human EF1a promoter CDS 4617..5015 /label=Bsd RBS 4009..4593 /label=IRES CDS 3369..3998 /label=human Lin28 BASE COUNT 2230 a 2283 c 2455 g 2210 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccgccaccat gggctccgtg tccaaccagc agtttgcagg tggctgcgcc aaggcggcag 3421 aagaggcgcc cgaggaggcg ccggaggacg cggcccgggc ggcggacgag cctcagctgc 3481 tgcacggtgc gggcatctgt aagtggttca acgtgcgcat ggggttcggc ttcctgtcca 3541 tgaccgcccg cgccggggtc gcgctcgacc ccccagtgga tgtctttgtg caccagagta 3601 agctgcacat ggaagggttc cggagcttga aggagggtga ggcagtggag ttcaccttta 3661 agaagtcagc caagggtctg gaatccatcc gtgtcaccgg acctggtgga gtattctgta 3721 ttgggagtga gaggcggcca aaaggaaaga gcatgcagaa gcgcagatca aaaggagaca 3781 ggtgctacaa ctgtggaggt ctagatcatc atgccaagga atgcaagctg ccaccccagc 3841 ccaagaagtg ccacttctgc cagagcatca gccatatggt agcctcatgt ccgctgaagg 3901 cccagcaggg ccctagtgca cagggaaagc caacctactt tcgagaggaa gaagaagaaa 3961 tccacagccc taccctgctc ccggaggcac agaattgagc cacagctagc ccctctccct 4021 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct 4081 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc 4141 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc 4201 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg 4261 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa 4321 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt 4381 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg 4441 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta 4501 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt 4561 tcctttgaaa aacacgatga taatatggcc acactagaga tccaccggtc gccaccatgg 4621 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca 4681 gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca 4741 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc 4801 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg 4861 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc 4921 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg 4981 aattgctgcc ctctggttat gtgtgggagg gctaagcggc cgcgtcgaca atcaacctct 5041 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 5101 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 5161 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 5221 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 5281 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 5341 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 5401 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc 5461 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 5521 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 5581 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct 5641 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 5701 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg 5761 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 5821 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 5881 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 5941 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 6001 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 6061 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 6121 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc 6181 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 6241 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 6301 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc 6361 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 6421 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 6481 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc 6541 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 6601 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 6661 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 6721 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 6781 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 6841 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 6901 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct 6961 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 7021 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 7081 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 7141 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 7201 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 7261 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 7321 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 7381 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 7441 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 7501 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 7561 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 7621 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 7681 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 7741 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 7801 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 7861 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 7921 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 7981 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 8041 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 8101 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 8161 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 8221 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 8281 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 8341 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 8401 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 8461 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 8521 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 8581 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 8641 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 8701 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 8761 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 8821 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 8881 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 8941 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 9001 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 9061 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 9121 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgc //