LOCUS       pLV-EF1a-hNanog-IRES-Puro   9667 bp    DNA     circular      1-FEB-2008
COMMENT     
COMMENT     This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             7588..8448
                     /label=Amp(R)
     promoter        8649..9189
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6193..6427
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7002..7457)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    6630..6843
                     /label=SV40 ori
     promoter        1933..3322
                     /label=human EF1a promoter
     CDS             4905..5504
                     /label=Puro
     RBS             4297..4881
                     /label=IRES
     CDS             3369..4286
                     /label=human Nanog
BASE COUNT     2345 a      2497 c      2536 g      2289 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat gagtgtggac ccagcttgtc cccaaagctt gccttgcttt gaagcatccg 
     3421 actgtaaaga atcttcacct atgcctgtga tttgtgggcc tgaagaaaac tatccatcct 
     3481 tgcaaatgtc ttctgctgag atgcctcaca cggagactgt ctctcctctt ccttcctcca 
     3541 tggatctgct tattcaggac agccctgatt cttccaccag tcccaaaggc aaacaaccca 
     3601 cttctgcaga gaagagtgtc gcaaaaaagg aagacaaggt cccggtcaag aaacagaaga 
     3661 ccagaactgt gttctcttcc acccagctgt gtgtactcaa tgatagattt cagagacaga 
     3721 aatacctcag cctccagcag atgcaagaac tctccaacat cctgaacctc agctacaaac 
     3781 aggtgaagac ctggttccag aaccagagaa tgaaatctaa gaggtggcag aaaaacaact 
     3841 ggccgaagaa tagcaatggt gtgacgcaga aggcctcagc acctacctac cccagccttt 
     3901 actcttccta ccaccaggga tgcctggtga acccgactgg gaaccttcca atgtggagca 
     3961 accagacctg gaacaattca acctggagca accagaccca gaacatccag tcctggagca 
     4021 accactcctg gaacactcag acctggtgca cccaatcctg gaacaatcag gcctggaaca 
     4081 gtcccttcta taactgtgga gaggaatctc tgcagtcctg catgcagttc cagccaaatt 
     4141 ctcctgccag tgacttggag gctgccttgg aagctgctgg ggaaggcctt aatgtaatac 
     4201 agcagaccac taggtatttt agtactccac aaaccatgga tttattccta aactactcca 
     4261 tgaacatgca acctgaagac gtgtgaagat gagctagccc ctctccctcc ccccccccta 
     4321 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt 
     4381 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga 
     4441 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg 
     4501 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt 
     4561 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat 
     4621 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg 
     4681 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg 
     4741 taccccattg tatgggatct gatctggggc ctcggtacac atgctttaca tgtgtttagt 
     4801 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa 
     4861 cacgatgata atatggccac actagagatc caccggtcgc caccatgacc gagtacaagc 
     4921 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 
     4981 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 
     5041 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 
     5101 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 
     5161 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 
     5221 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 
     5281 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 
     5341 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 
     5401 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg 
     5461 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagcggcc gcgtcgacaa 
     5521 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 
     5581 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 
     5641 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 
     5701 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 
     5761 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 
     5821 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 
     5881 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 
     5941 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 
     6001 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 
     6061 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc 
     6121 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 
     6181 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct 
     6241 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 
     6301 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 
     6361 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 
     6421 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 
     6481 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 
     6541 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 
     6601 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 
     6661 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 
     6721 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 
     6781 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg 
     6841 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 
     6901 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 
     6961 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 
     7021 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 
     7081 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 
     7141 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 
     7201 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 
     7261 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 
     7321 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 
     7381 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 
     7441 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 
     7501 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 
     7561 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 
     7621 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 
     7681 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 
     7741 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 
     7801 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 
     7861 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 
     7921 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 
     7981 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 
     8041 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 
     8101 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 
     8161 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 
     8221 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 
     8281 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 
     8341 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 
     8401 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 
     8461 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 
     8521 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 
     8581 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 
     8641 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 
     8701 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 
     8761 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 
     8821 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 
     8881 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 
     8941 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 
     9001 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 
     9061 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 
     9121 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 
     9181 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 
     9241 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 
     9301 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 
     9361 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 
     9421 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 
     9481 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 
     9541 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 
     9601 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg 
     9661 gagctgc 
//
