LOCUS pLV-EF1a-hNanog-IRES-Bsd 9466 bp DNA circular 12-MAR-2008 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7387..8247 /label=Amp(R) promoter 8448..8988 /label=pUC ori promoter 7..235 /label=RSV LTR 5992..6226 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(6801..7256) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 6429..6642 /label=SV40 ori promoter 1933..3322 /label=human EF1a promoter CDS 4905..5303 /label=Bsd RBS 4297..4881 /label=IRES CDS 3369..4286 /label=huamn Nanog BASE COUNT 2347 a 2368 c 2446 g 2305 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccgccaccat gagtgtggac ccagcttgtc cccaaagctt gccttgcttt gaagcatccg 3421 actgtaaaga atcttcacct atgcctgtga tttgtgggcc tgaagaaaac tatccatcct 3481 tgcaaatgtc ttctgctgag atgcctcaca cggagactgt ctctcctctt ccttcctcca 3541 tggatctgct tattcaggac agccctgatt cttccaccag tcccaaaggc aaacaaccca 3601 cttctgcaga gaagagtgtc gcaaaaaagg aagacaaggt cccggtcaag aaacagaaga 3661 ccagaactgt gttctcttcc acccagctgt gtgtactcaa tgatagattt cagagacaga 3721 aatacctcag cctccagcag atgcaagaac tctccaacat cctgaacctc agctacaaac 3781 aggtgaagac ctggttccag aaccagagaa tgaaatctaa gaggtggcag aaaaacaact 3841 ggccgaagaa tagcaatggt gtgacgcaga aggcctcagc acctacctac cccagccttt 3901 actcttccta ccaccaggga tgcctggtga acccgactgg gaaccttcca atgtggagca 3961 accagacctg gaacaattca acctggagca accagaccca gaacatccag tcctggagca 4021 accactcctg gaacactcag acctggtgca cccaatcctg gaacaatcag gcctggaaca 4081 gtcccttcta taactgtgga gaggaatctc tgcagtcctg catgcagttc cagccaaatt 4141 ctcctgccag tgacttggag gctgccttgg aagctgctgg ggaaggcctt aatgtaatac 4201 agcagaccac taggtatttt agtactccac aaaccatgga tttattccta aactactcca 4261 tgaacatgca acctgaagac gtgtgaagat gagctagccc ctctccctcc ccccccccta 4321 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt 4381 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga 4441 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg 4501 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt 4561 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat 4621 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg 4681 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg 4741 taccccattg tatgggatct gatctggggc ctcggtacac atgctttaca tgtgtttagt 4801 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa 4861 cacgatgata atatggccac actagagatc caccggtcgc caccatggcc aagcctttgt 4921 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct 4981 ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg 5041 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg 5101 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca 5161 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag 5221 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct 5281 ctggttatgt gtgggagggc taagcggccg cgtcgacaat caacctctgg attacaaaat 5341 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 5401 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 5461 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 5521 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 5581 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 5641 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 5701 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 5761 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 5821 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 5881 gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt aagaccaatg 5941 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg 6001 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta 6061 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 6121 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 6181 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 6241 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac 6301 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 6361 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 6421 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca 6481 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 6541 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga 6601 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca 6661 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 6721 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 6781 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 6841 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 6901 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 6961 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 7021 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 7081 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 7141 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 7201 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt 7261 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 7321 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 7381 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 7441 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 7501 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 7561 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 7621 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 7681 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 7741 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 7801 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 7861 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 7921 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 7981 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 8041 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 8101 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 8161 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 8221 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 8281 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 8341 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 8401 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 8461 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 8521 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 8581 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 8641 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 8701 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 8761 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 8821 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 8881 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 8941 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 9001 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 9061 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 9121 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 9181 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 9241 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 9301 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 9361 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc 9421 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgc //