LOCUS       pLV-EF1a-hSox2-IRES-Neo    9888 bp    DNA     circular     13-SEP-2009
COMMENT     
COMMENT     This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             7809..8669
                     /label=Amp(R)
     promoter        8870..9410
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6414..6648
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7223..7678)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    6851..7064
                     /label=SV40 ori
     promoter        1933..3322
                     /label=human EF1a promoter
     RBS             4335..4919
                     /label=IRES
     CDS             3369..4322
                     /label=human Sox2
     CDS             4931..5725
                     /label=Neo
BASE COUNT     2334 a      2552 c      2688 g      2314 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat gtacaacatg atggagacgg agctgaagcc gccgggcccg cagcaaactt 
     3421 cggggggcgg cggcggcaac tccaccgcgg cggcggccgg cggcaaccag aaaaacagcc 
     3481 cggaccgcgt caagcggccc atgaatgcct tcatggtgtg gtcccgcggg cagcggcgca 
     3541 agatggccca ggagaacccc aagatgcaca actcggagat cagcaagcgc ctgggcgccg 
     3601 agtggaaact tttgtcggag acggagaagc ggccgttcat cgacgaggct aagcggctgc 
     3661 gagcgctgca catgaaggag cacccggatt ataaataccg gccccggcgg aaaaccaaga 
     3721 cgctcatgaa gaaggataag tacacgctgc ccggcgggct gctggccccc ggcggcaata 
     3781 gcatggcgag cggggtcggg gtgggcgccg gcctgggcgc gggcgtgaac cagcgcatgg 
     3841 acagttacgc gcacatgaac ggctggagca acggcagcta cagcatgatg caggaccagc 
     3901 tgggctaccc gcagcacccg ggcctcaatg cgcacggcgc agcgcagatg cagcccatgc 
     3961 accgctacga cgtgagcgcc ctgcagtaca actccatgac cagctcgcag acctacatga 
     4021 acggctcgcc cacctacagc atgtcctact cgcagcaggg cacccctggc atggctcttg 
     4081 gctccatggg ttcggtggtc aagtccgagg ccagctccag cccccctgtg gttacctctt 
     4141 cctcccactc cagggcgccc tgccaggccg gggacctccg ggacatgatc agcatgtatc 
     4201 tccccggcgc cgaggtgccg gaacccgccg cccccagcag acttcacatg tcccagcact 
     4261 accagagcgg cccggtgccc ggcacggcca ttaacggcac actgcccctc tcacacatgt 
     4321 gagggccgga gctagcccct ctccctcccc cccccctaac gttactggcc gaagccgctt 
     4381 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg 
     4441 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc 
     4501 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga 
     4561 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc 
     4621 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc 
     4681 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc 
     4741 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga 
     4801 tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg 
     4861 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac 
     4921 tagagccacc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 
     4981 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 
     5041 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 
     5101 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 
     5161 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 
     5221 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 
     5281 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 
     5341 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 
     5401 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 
     5461 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 
     5521 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 
     5581 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 
     5641 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 
     5701 tcgccttctt gacgagttct tctgagcggc cgcgtcgaca atcaacctct ggattacaaa 
     5761 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 
     5821 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 
     5881 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 
     5941 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 
     6001 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 
     6061 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 
     6121 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt 
     6181 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 
     6241 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 
     6301 cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa 
     6361 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag 
     6421 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt 
     6481 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 
     6541 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 
     6601 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat 
     6661 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga 
     6721 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 
     6781 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 
     6841 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc 
     6901 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 
     6961 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 
     7021 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta 
     7081 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc 
     7141 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 
     7201 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 
     7261 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 
     7321 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 
     7381 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 
     7441 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 
     7501 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 
     7561 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 
     7621 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag 
     7681 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     7741 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     7801 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 
     7861 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 
     7921 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 
     7981 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 
     8041 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 
     8101 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 
     8161 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 
     8221 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 
     8281 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 
     8341 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 
     8401 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 
     8461 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 
     8521 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 
     8581 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 
     8641 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 
     8701 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 
     8761 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     8821 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     8881 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     8941 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 
     9001 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     9061 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     9121 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     9181 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     9241 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     9301 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     9361 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     9421 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     9481 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 
     9541 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 
     9601 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 
     9661 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 
     9721 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 
     9781 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 
     9841 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgc 
//
