LOCUS       pLV-EF1a-hSox2-IRES-Hyg  10131 bp    DNA     circular     13-SEP-2009
COMMENT     
COMMENT     This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             8052..8912
                     /label=Amp(R)
     promoter        9113..9653
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6657..6891
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7466..7921)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    7094..7307
                     /label=SV40 ori
     promoter        1933..3322
                     /label=human EF1a promoter
     RBS             4335..4919
                     /label=IRES
     CDS             3369..4322
                     /label=human Sox2
     CDS             4943..5968
                     /label=Hyg
BASE COUNT     2403 a      2614 c      2756 g      2358 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat gtacaacatg atggagacgg agctgaagcc gccgggcccg cagcaaactt 
     3421 cggggggcgg cggcggcaac tccaccgcgg cggcggccgg cggcaaccag aaaaacagcc 
     3481 cggaccgcgt caagcggccc atgaatgcct tcatggtgtg gtcccgcggg cagcggcgca 
     3541 agatggccca ggagaacccc aagatgcaca actcggagat cagcaagcgc ctgggcgccg 
     3601 agtggaaact tttgtcggag acggagaagc ggccgttcat cgacgaggct aagcggctgc 
     3661 gagcgctgca catgaaggag cacccggatt ataaataccg gccccggcgg aaaaccaaga 
     3721 cgctcatgaa gaaggataag tacacgctgc ccggcgggct gctggccccc ggcggcaata 
     3781 gcatggcgag cggggtcggg gtgggcgccg gcctgggcgc gggcgtgaac cagcgcatgg 
     3841 acagttacgc gcacatgaac ggctggagca acggcagcta cagcatgatg caggaccagc 
     3901 tgggctaccc gcagcacccg ggcctcaatg cgcacggcgc agcgcagatg cagcccatgc 
     3961 accgctacga cgtgagcgcc ctgcagtaca actccatgac cagctcgcag acctacatga 
     4021 acggctcgcc cacctacagc atgtcctact cgcagcaggg cacccctggc atggctcttg 
     4081 gctccatggg ttcggtggtc aagtccgagg ccagctccag cccccctgtg gttacctctt 
     4141 cctcccactc cagggcgccc tgccaggccg gggacctccg ggacatgatc agcatgtatc 
     4201 tccccggcgc cgaggtgccg gaacccgccg cccccagcag acttcacatg tcccagcact 
     4261 accagagcgg cccggtgccc ggcacggcca ttaacggcac actgcccctc tcacacatgt 
     4321 gagggccgga gctagcccct ctccctcccc cccccctaac gttactggcc gaagccgctt 
     4381 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg 
     4441 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc 
     4501 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga 
     4561 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc 
     4621 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc 
     4681 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc 
     4741 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga 
     4801 tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg 
     4861 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac 
     4921 tagagatcca ccggtcgcca ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa 
     4981 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga 
     5041 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg 
     5101 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc 
     5161 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg 
     5221 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca 
     5281 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt 
     5341 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc 
     5401 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc 
     5461 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca 
     5521 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc 
     5581 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt 
     5641 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca 
     5701 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca 
     5761 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg 
     5821 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag 
     5881 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc 
     5941 cagcactcgt ccgagggcaa aggaataggc ggccgcgtcg acaatcaacc tctggattac 
     6001 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 
     6061 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 
     6121 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 
     6181 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 
     6241 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 
     6301 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 
     6361 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg 
     6421 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 
     6481 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg 
     6541 agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta cctttaagac 
     6601 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 
     6661 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct 
     6721 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 
     6781 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 
     6841 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 
     6901 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 
     6961 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 
     7021 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 
     7081 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc 
     7141 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 
     7201 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 
     7261 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt 
     7321 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat 
     7381 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag 
     7441 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt 
     7501 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 
     7561 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 
     7621 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 
     7681 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 
     7741 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 
     7801 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 
     7861 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt 
     7921 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 
     7981 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 
     8041 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 
     8101 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 
     8161 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 
     8221 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 
     8281 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 
     8341 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 
     8401 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 
     8461 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 
     8521 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 
     8581 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 
     8641 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 
     8701 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 
     8761 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 
     8821 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 
     8881 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 
     8941 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 
     9001 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
     9061 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
     9121 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
     9181 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 
     9241 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
     9301 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
     9361 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
     9421 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
     9481 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
     9541 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
     9601 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
     9661 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
     9721 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 
     9781 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 
     9841 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 
     9901 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 
     9961 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 
    10021 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 
    10081 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg c 
//
