LOCUS       pLV-EF1a-hSox2-IRES-Bsd    9504 bp    DNA     circular     12-MAR-2008
COMMENT     
COMMENT     This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             7425..8285
                     /label=Amp(R)
     promoter        8486..9026
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6030..6264
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6839..7294)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    6467..6680
                     /label=SV40 ori
     promoter        1933..3322
                     /label=human EF1a promoter
     CDS             4943..5341
                     /label=Bsd
     RBS             4335..4919
                     /label=IRES
     CDS             3369..4322
                     /label=human Sox2
BASE COUNT     2281 a      2437 c      2556 g      2230 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat gtacaacatg atggagacgg agctgaagcc gccgggcccg cagcaaactt 
     3421 cggggggcgg cggcggcaac tccaccgcgg cggcggccgg cggcaaccag aaaaacagcc 
     3481 cggaccgcgt caagcggccc atgaatgcct tcatggtgtg gtcccgcggg cagcggcgca 
     3541 agatggccca ggagaacccc aagatgcaca actcggagat cagcaagcgc ctgggcgccg 
     3601 agtggaaact tttgtcggag acggagaagc ggccgttcat cgacgaggct aagcggctgc 
     3661 gagcgctgca catgaaggag cacccggatt ataaataccg gccccggcgg aaaaccaaga 
     3721 cgctcatgaa gaaggataag tacacgctgc ccggcgggct gctggccccc ggcggcaata 
     3781 gcatggcgag cggggtcggg gtgggcgccg gcctgggcgc gggcgtgaac cagcgcatgg 
     3841 acagttacgc gcacatgaac ggctggagca acggcagcta cagcatgatg caggaccagc 
     3901 tgggctaccc gcagcacccg ggcctcaatg cgcacggcgc agcgcagatg cagcccatgc 
     3961 accgctacga cgtgagcgcc ctgcagtaca actccatgac cagctcgcag acctacatga 
     4021 acggctcgcc cacctacagc atgtcctact cgcagcaggg cacccctggc atggctcttg 
     4081 gctccatggg ttcggtggtc aagtccgagg ccagctccag cccccctgtg gttacctctt 
     4141 cctcccactc cagggcgccc tgccaggccg gggacctccg ggacatgatc agcatgtatc 
     4201 tccccggcgc cgaggtgccg gaacccgccg cccccagcag acttcacatg tcccagcact 
     4261 accagagcgg cccggtgccc ggcacggcca ttaacggcac actgcccctc tcacacatgt 
     4321 gagggccgga gctagcccct ctccctcccc cccccctaac gttactggcc gaagccgctt 
     4381 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg 
     4441 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc 
     4501 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga 
     4561 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc 
     4621 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc 
     4681 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc 
     4741 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga 
     4801 tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg 
     4861 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac 
     4921 tagagatcca ccggtcgcca ccatggccaa gcctttgtct caagaagaat ccaccctcat 
     4981 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag 
     5041 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg 
     5101 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct 
     5161 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg 
     5221 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg 
     5281 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta 
     5341 agcggccgcg tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 
     5401 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 
     5461 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 
     5521 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 
     5581 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 
     5641 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 
     5701 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc 
     5761 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 
     5821 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 
     5881 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 
     5941 ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc 
     6001 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac 
     6061 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 
     6121 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 
     6181 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 
     6241 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 
     6301 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg 
     6361 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 
     6421 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc 
     6481 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 
     6541 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 
     6601 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt 
     6661 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 
     6721 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 
     6781 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac 
     6841 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 
     6901 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 
     6961 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 
     7021 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 
     7081 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 
     7141 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 
     7201 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 
     7261 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc 
     7321 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 
     7381 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 
     7441 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 
     7501 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 
     7561 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 
     7621 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 
     7681 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 
     7741 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 
     7801 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 
     7861 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 
     7921 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 
     7981 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 
     8041 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 
     8101 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 
     8161 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 
     8221 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 
     8281 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 
     8341 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 
     8401 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 
     8461 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 
     8521 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 
     8581 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 
     8641 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 
     8701 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 
     8761 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 
     8821 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 
     8881 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 
     8941 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 
     9001 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 
     9061 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 
     9121 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 
     9181 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca 
     9241 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 
     9301 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 
     9361 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 
     9421 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact 
     9481 aaagggaaca aaagctggag ctgc 
//
