LOCUS       pLV-EF1a-hOct4-IRES-Hyg   10263 bp    DNA     circular     12-MAR-2008
COMMENT     
COMMENT     This file is created by Biosettia Inc
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     CDS             8184..9044
                     /label=Amp(R)
     promoter        9245..9785
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6789..7023
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7598..8053)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    7226..7439
                     /label=SV40 ori
     promoter        1933..3322
                     /label=human EF1a promoter
     RBS             4467..5051
                     /label=IRES
     CDS             3369..4451
                     /label=human Oct4
     CDS             5075..6100
                     /label=Hyg
BASE COUNT     2428 a      2594 c      2798 g      2443 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgccaccat ggcgggacac ctggcttcgg atttcgcctt ctcgccccct ccaggtggtg 
     3421 gaggtgatgg gccagggggg ccggagccgg gctgggttga tcctcggacc tggctaagct 
     3481 tccaaggccc tcctggaggg ccaggaatcg ggccgggggt tgggccaggc tctgaggtgt 
     3541 gggggattcc cccatgcccc ccgccgtatg agttctgtgg ggggatggcg tactgtgggc 
     3601 cccaggttgg agtggggcta gtgccccaag gcggcttgga gacctctcag cctgagggtg 
     3661 aagcaggagt cggggtggag agcaactccg atggggcctc cccggagccc tgcaccgtca 
     3721 cccctggtgc cgtgaagctg gagaaggaga agctggagca aaacccggag gagtcccagg 
     3781 acatcaaagc tctgcagaaa gaactcgagc aatttgccaa gctcctgaag cagaagagga 
     3841 tcaccctggg atatacacag gccgatgtgg ggctcaccct gggggttcta tttgggaagg 
     3901 tattcagcca aacgaccatc tgccgctttg aggctctgca gcttagcttc aagaacatgt 
     3961 gtaagctgcg gcccttgctg cagaagtggg tggaggaagc tgacaacaat gaaaatcttc 
     4021 aggagatatg caaagcagaa accctcgtgc aggcccgaaa gagaaagcga accagtatcg 
     4081 agaaccgagt gagaggcaac ctggagaatt tgttcctgca gtgcccgaaa cccacactgc 
     4141 agcagatcag ccacatcgcc cagcagcttg ggctcgagaa ggatgtggtc cgagtgtggt 
     4201 tctgtaaccg gcgccagaag ggcaagcgat caagcagcga ctatgcacaa cgagaggatt 
     4261 ttgaggctgc tgggtctcct ttctcagggg gaccagtgtc ctttcctctg gccccagggc 
     4321 cccattttgg taccccaggc tatgggagcc ctcacttcac tgcactgtac tcctcggtcc 
     4381 ctttccctga gggggaagcc tttccccctg tctccgtcac cactctgggc tctcccatgc 
     4441 attcaaactg aggtgcctgc ccgctagccc ctctccctcc ccccccccta acgttactgg 
     4501 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt 
     4561 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc 
     4621 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc 
     4681 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg 
     4741 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc 
     4801 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa 
     4861 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg 
     4921 tatgggatct gatctggggc ctcggtacac atgctttaca tgtgtttagt cgaggttaaa 
     4981 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata 
     5041 atatggccac actagagatc caccggtcgc caccatgaaa aagcctgaac tcaccgcgac 
     5101 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc 
     5161 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg 
     5221 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc 
     5281 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta 
     5341 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc 
     5401 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca 
     5461 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga 
     5521 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac 
     5581 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc 
     5641 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg 
     5701 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt 
     5761 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt 
     5821 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat 
     5881 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc 
     5941 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat 
     6001 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg 
     6061 aaaccgacgc cccagcactc gtccgagggc aaaggaatag gcggccgcgt cgacaatcaa 
     6121 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 
     6181 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 
     6241 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 
     6301 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 
     6361 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 
     6421 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 
     6481 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 
     6541 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 
     6601 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 
     6661 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcgg 
     6721 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 
     6781 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta 
     6841 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 
     6901 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 
     6961 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 
     7021 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 
     7081 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 
     7141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 
     7201 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc 
     7261 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 
     7321 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 
     7381 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca 
     7441 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 
     7501 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 
     7561 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta 
     7621 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 
     7681 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 
     7741 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 
     7801 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 
     7861 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 
     7921 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 
     7981 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 
     8041 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 
     8101 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 
     8161 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 
     8221 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 
     8281 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 
     8341 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 
     8401 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 
     8461 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 
     8521 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 
     8581 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 
     8641 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 
     8701 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 
     8761 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 
     8821 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 
     8881 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 
     8941 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 
     9001 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 
     9061 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 
     9121 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 
     9181 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 
     9241 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 
     9301 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 
     9361 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 
     9421 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 
     9481 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 
     9541 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 
     9601 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 
     9661 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 
     9721 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 
     9781 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 
     9841 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 
     9901 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 
     9961 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 
    10021 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 
    10081 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 
    10141 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 
    10201 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc 
    10261 tgc 
//
