LOCUS pLV-EF1a-hOct4-IRES-Bsd 9636 bp DNA circular 12-MAR-2008 COMMENT COMMENT This file is created by Biosettia Inc http://www.biosettia.com/ FEATURES Location/Qualifiers CDS 7557..8417 /label=Amp(R) promoter 8618..9158 /label=pUC ori promoter 7..235 /label=RSV LTR 6162..6396 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(6971..7426) /label=f1 ori LTR 236..416 /label=5'LTR misc_feature 6599..6812 /label=SV40 ori promoter 1933..3322 /label=human EF1a promoter CDS 5075..5473 /label=Bsd RBS 4467..5051 /label=IRES CDS 3369..4451 /label=human Oct4 BASE COUNT 2306 a 2417 c 2598 g 2315 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccgccaccat ggcgggacac ctggcttcgg atttcgcctt ctcgccccct ccaggtggtg 3421 gaggtgatgg gccagggggg ccggagccgg gctgggttga tcctcggacc tggctaagct 3481 tccaaggccc tcctggaggg ccaggaatcg ggccgggggt tgggccaggc tctgaggtgt 3541 gggggattcc cccatgcccc ccgccgtatg agttctgtgg ggggatggcg tactgtgggc 3601 cccaggttgg agtggggcta gtgccccaag gcggcttgga gacctctcag cctgagggtg 3661 aagcaggagt cggggtggag agcaactccg atggggcctc cccggagccc tgcaccgtca 3721 cccctggtgc cgtgaagctg gagaaggaga agctggagca aaacccggag gagtcccagg 3781 acatcaaagc tctgcagaaa gaactcgagc aatttgccaa gctcctgaag cagaagagga 3841 tcaccctggg atatacacag gccgatgtgg ggctcaccct gggggttcta tttgggaagg 3901 tattcagcca aacgaccatc tgccgctttg aggctctgca gcttagcttc aagaacatgt 3961 gtaagctgcg gcccttgctg cagaagtggg tggaggaagc tgacaacaat gaaaatcttc 4021 aggagatatg caaagcagaa accctcgtgc aggcccgaaa gagaaagcga accagtatcg 4081 agaaccgagt gagaggcaac ctggagaatt tgttcctgca gtgcccgaaa cccacactgc 4141 agcagatcag ccacatcgcc cagcagcttg ggctcgagaa ggatgtggtc cgagtgtggt 4201 tctgtaaccg gcgccagaag ggcaagcgat caagcagcga ctatgcacaa cgagaggatt 4261 ttgaggctgc tgggtctcct ttctcagggg gaccagtgtc ctttcctctg gccccagggc 4321 cccattttgg taccccaggc tatgggagcc ctcacttcac tgcactgtac tcctcggtcc 4381 ctttccctga gggggaagcc tttccccctg tctccgtcac cactctgggc tctcccatgc 4441 attcaaactg aggtgcctgc ccgctagccc ctctccctcc ccccccccta acgttactgg 4501 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt 4561 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc 4621 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc 4681 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg 4741 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc 4801 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa 4861 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg 4921 tatgggatct gatctggggc ctcggtacac atgctttaca tgtgtttagt cgaggttaaa 4981 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata 5041 atatggccac actagagatc caccggtcgc caccatggcc aagcctttgt ctcaagaaga 5101 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta 5161 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata 5221 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc 5281 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc 5341 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa 5401 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt 5461 gtgggagggc taagcggccg cgtcgacaat caacctctgg attacaaaat ttgtgaaaga 5521 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 5581 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 5641 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 5701 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 5761 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 5821 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 5881 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 5941 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 6001 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 6061 tgggccgcct ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg 6121 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 6181 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 6241 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 6301 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 6361 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 6421 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 6481 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 6541 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 6601 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 6661 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 6721 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat 6781 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac 6841 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 6901 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 6961 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 7021 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 7081 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 7141 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 7201 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 7261 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 7321 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 7381 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc 7441 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 7501 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 7561 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 7621 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 7681 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 7741 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 7801 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 7861 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 7921 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 7981 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 8041 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 8101 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 8161 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 8221 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 8281 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 8341 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 8401 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 8461 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 8521 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 8581 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 8641 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 8701 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 8761 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 8821 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 8881 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 8941 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 9001 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 9061 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 9121 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 9181 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 9241 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 9301 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 9361 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 9421 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 9481 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 9541 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa 9601 ttaaccctca ctaaagggaa caaaagctgg agctgc //