LOCUS       pLV-EF1a-RFP-IRES-Neo      9660 bp    DNA     circular     22-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7581..8441
                     /label=Amp(R)
     promoter        8642..9182
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6186..6420
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6995..7450)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6492..6622
                     /label=SV40 pA
     misc_feature    6623..6836
                     /label=SV40 ori
     misc_feature    5507..6103
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             4703..5497
                     /label=Neo
     RBS             4107..4691
                     /label=IRES
     CDS             3375..4085
                     /label=RFP
BASE COUNT     2306 a      2456 c      2605 g      2293 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgatatcgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt 
     3421 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg 
     3481 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg 
     3541 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg 
     3601 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct 
     3661 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact 
     3721 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct 
     3781 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt 
     3841 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg 
     3901 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg 
     3961 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg 
     4021 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca 
     4081 agtaaccaaa aaaatggcgg ccgctagccc ctctccctcc ccccccccta acgttactgg 
     4141 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt 
     4201 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc 
     4261 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc 
     4321 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg 
     4381 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc 
     4441 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa 
     4501 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg 
     4561 tatgggatct gatctggggc ctcggtacac atgctttaca tgtgtttagt cgaggttaaa 
     4621 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata 
     4681 atatggccac actagagcca ccatgattga acaagatgga ttgcacgcag gttctccggc 
     4741 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 
     4801 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 
     4861 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 
     4921 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 
     4981 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 
     5041 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 
     5101 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 
     5161 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 
     5221 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 
     5281 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 
     5341 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 
     5401 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 
     5461 catcgccttc tatcgccttc ttgacgagtt cttctgagcg gccgcgtcga caatcaacct 
     5521 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 
     5581 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 
     5641 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 
     5701 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 
     5761 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 
     5821 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 
     5881 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 
     5941 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 
     6001 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 
     6061 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac 
     6121 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 
     6181 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg 
     6241 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 
     6301 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 
     6361 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 
     6421 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 
     6481 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     6541 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 
     6601 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc 
     6661 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 
     6721 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 
     6781 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg 
     6841 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 
     6901 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 
     6961 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc 
     7021 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 
     7081 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 
     7141 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 
     7201 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 
     7261 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 
     7321 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 
     7381 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 
     7441 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 
     7501 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
     7561 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 
     7621 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 
     7681 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 
     7741 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 
     7801 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 
     7861 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 
     7921 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 
     7981 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 
     8041 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 
     8101 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 
     8161 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 
     8221 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 
     8281 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 
     8341 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 
     8401 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 
     8461 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 
     8521 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
     8581 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     8641 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     8701 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 
     8761 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     8821 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     8881 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     8941 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     9001 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     9061 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     9121 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     9181 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     9241 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 
     9301 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 
     9361 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 
     9421 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 
     9481 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 
     9541 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 
     9601 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc 
//
