LOCUS pLV-EF1a-RFP-IRES-Hyg 9903 bp DNA circular 22-JAN-2013 COMMENT COMMENT This file is created by Biosettia Inc. http://www.biosettia.com/ FEATURES Location/Qualifiers misc_feature 1807..1929 /label=cPPT CDS 7824..8684 /label=Amp(R) promoter 8885..9425 /label=pUC ori promoter 7..235 /label=RSV LTR 6429..6663 /label=3' LTR intron 526..1690 /label=Intron rep_origin complement(7238..7693) /label=f1 ori LTR 236..416 /label=5'LTR polyA_signal 6735..6865 /label=SV40 pA misc_feature 6866..7079 /label=SV40 ori misc_feature 5750..6346 /label=WPRE promoter 1933..3322 /label=human EF1a promoter intron 2361..3303 /label=intron CDS 4715..5740 /label=Hyg RBS 4107..4691 /label=IRES CDS 3375..4085 /label=RFP BASE COUNT 2375 a 2518 c 2673 g 2337 t ORIGIN 1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 3361 ccgatatcgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt 3421 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg 3481 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg 3541 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg 3601 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct 3661 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact 3721 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct 3781 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt 3841 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg 3901 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg 3961 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg 4021 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca 4081 agtaaccaaa aaaatggcgg ccgctagccc ctctccctcc ccccccccta acgttactgg 4141 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt 4201 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc 4261 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc 4321 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg 4381 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc 4441 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa 4501 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg 4561 tatgggatct gatctggggc ctcggtacac atgctttaca tgtgtttagt cgaggttaaa 4621 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata 4681 atatggccac actagagatc caccggtcgc caccatgaaa aagcctgaac tcaccgcgac 4741 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc 4801 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg 4861 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc 4921 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta 4981 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc 5041 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca 5101 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga 5161 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac 5221 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc 5281 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg 5341 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt 5401 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt 5461 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat 5521 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc 5581 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat 5641 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg 5701 aaaccgacgc cccagcactc gtccgagggc aaaggaatag gcggccgcgt cgacaatcaa 5761 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 5821 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 5881 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 5941 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 6001 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 6061 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 6121 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 6181 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 6241 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 6301 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcgg 6361 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 6421 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta 6481 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 6541 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 6601 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 6661 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 6721 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 6781 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 6841 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc 6901 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 6961 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 7021 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca 7081 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 7141 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 7201 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta 7261 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 7321 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 7381 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 7441 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 7501 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 7561 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 7621 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 7681 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 7741 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 7801 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 7861 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 7921 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 7981 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 8041 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 8101 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 8161 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 8221 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 8281 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 8341 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 8401 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 8461 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 8521 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 8581 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 8641 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 8701 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 8761 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 8821 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 8881 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 8941 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 9001 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 9061 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 9121 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 9181 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 9241 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 9301 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 9361 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 9421 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 9481 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 9541 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 9601 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 9661 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 9721 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 9781 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 9841 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc 9901 tgc //