LOCUS       pLV-EF1a-RFP-IRES-Bsd      9276 bp    DNA     circular     22-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7197..8057
                     /label=Amp(R)
     promoter        8258..8798
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5802..6036
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6611..7066)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6108..6238
                     /label=SV40 pA
     misc_feature    6239..6452
                     /label=SV40 ori
     misc_feature    5123..5719
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             4715..5113
                     /label=Bsd
     RBS             4107..4691
                     /label=IRES
     CDS             3375..4085
                     /label=RFP
BASE COUNT     2253 a      2341 c      2473 g      2209 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgatatcgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt 
     3421 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg 
     3481 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg 
     3541 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg 
     3601 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct 
     3661 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact 
     3721 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct 
     3781 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt 
     3841 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg 
     3901 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg 
     3961 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg 
     4021 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca 
     4081 agtaaccaaa aaaatggcgg ccgctagccc ctctccctcc ccccccccta acgttactgg 
     4141 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt 
     4201 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc 
     4261 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc 
     4321 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg 
     4381 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc 
     4441 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa 
     4501 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg 
     4561 tatgggatct gatctggggc ctcggtacac atgctttaca tgtgtttagt cgaggttaaa 
     4621 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata 
     4681 atatggccac actagagatc caccggtcgc caccatggcc aagcctttgt ctcaagaaga 
     4741 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta 
     4801 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata 
     4861 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc 
     4921 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc 
     4981 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa 
     5041 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt 
     5101 gtgggagggc taagcggccg cgtcgacaat caacctctgg attacaaaat ttgtgaaaga 
     5161 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 
     5221 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 
     5281 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 
     5341 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 
     5401 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 
     5461 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 
     5521 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 
     5581 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 
     5641 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 
     5701 tgggccgcct ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg 
     5761 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 
     5821 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 
     5881 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 
     5941 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 
     6001 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 
     6061 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 
     6121 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 
     6181 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 
     6241 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     6301 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 
     6361 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat 
     6421 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac 
     6481 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 
     6541 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 
     6601 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 
     6661 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 
     6721 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 
     6781 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 
     6841 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 
     6901 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 
     6961 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 
     7021 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc 
     7081 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 
     7141 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 
     7201 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 
     7261 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 
     7321 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 
     7381 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 
     7441 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 
     7501 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 
     7561 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 
     7621 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 
     7681 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 
     7741 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 
     7801 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 
     7861 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 
     7921 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 
     7981 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 
     8041 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 
     8101 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 
     8161 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     8221 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     8281 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     8341 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 
     8401 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     8461 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     8521 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     8581 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     8641 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     8701 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     8761 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     8821 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 
     8881 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 
     8941 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 
     9001 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 
     9061 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 
     9121 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 
     9181 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa 
     9241 ttaaccctca ctaaagggaa caaaagctgg agctgc 
//
