LOCUS       pLV-EF1a-RFP-Bsd        8637 bp    DNA     circular     22-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             6558..7418
                     /label=Amp(R)
     promoter        7619..8159
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5163..5397
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(5972..6427)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5469..5599
                     /label=SV40 pA
     misc_feature    5600..5813
                     /label=SV40 ori
     misc_feature    4484..5080
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             3350..4057
                     /label=RFP
     CDS             4076..4474
                     /label=Bsd
BASE COUNT     2096 a      2171 c      2312 g      2058 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagagt cgagctaccg gtcgccacca tggtgagcaa 
     3361 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga 
     3421 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga 
     3481 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga 
     3541 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat 
     3601 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt 
     3661 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat 
     3721 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa 
     3781 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg 
     3841 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac 
     3901 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt 
     3961 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg 
     4021 ccgccactcc accggcggca tggacgagct gtacaaggac ccaccggtcg ccaccatggc 
     4081 caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag 
     4141 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat 
     4201 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct 
     4261 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga 
     4321 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc 
     4381 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga 
     4441 attgctgccc tctggttatg tgtgggaggg ctaagcggcc gcgtcgacaa tcaacctctg 
     4501 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 
     4561 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 
     4621 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 
     4681 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 
     4741 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 
     4801 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 
     4861 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 
     4921 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 
     4981 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 
     5041 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt 
     5101 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 
     5161 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt 
     5221 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 
     5281 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 
     5341 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 
     5401 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 
     5461 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 
     5521 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 
     5581 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct 
     5641 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 
     5701 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 
     5761 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc 
     5821 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 
     5881 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 
     5941 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg 
     6001 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 
     6061 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 
     6121 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 
     6181 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 
     6241 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 
     6301 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 
     6361 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt 
     6421 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 
     6481 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 
     6541 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 
     6601 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 
     6661 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 
     6721 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 
     6781 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 
     6841 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 
     6901 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 
     6961 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 
     7021 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 
     7081 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 
     7141 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 
     7201 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 
     7261 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 
     7321 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 
     7381 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 
     7441 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 
     7501 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 
     7561 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 
     7621 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 
     7681 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 
     7741 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 
     7801 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 
     7861 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 
     7921 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 
     7981 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 
     8041 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 
     8101 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 
     8161 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 
     8221 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 
     8281 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 
     8341 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 
     8401 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 
     8461 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 
     8521 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc 
     8581 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgc 
//
