LOCUS       pLV-EF1a-GFP-Puro        8844 bp    DNA     circular     22-JAN-2013

COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             6765..7625
                     /label=Amp(R)
     promoter        7826..8366
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5370..5604
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6179..6634)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5676..5806
                     /label=SV40 pA
     misc_feature    5807..6020
                     /label=SV40 ori
     misc_feature    4691..5287
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             3347..4063
                     /label=GFP
     CDS             4082..4681
                     /label=Puro
BASE COUNT     2103 a      2315 c      2383 g      2043 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagaga tccaccggtc gccaccatgg tgagcaaggg 
     3361 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 
     3421 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 
     3481 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 
     3541 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 
     3601 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 
     3661 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 
     3721 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 
     3781 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 
     3841 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 
     3901 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 
     3961 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 
     4021 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aaggacccac cggtcgccac 
     4081 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 
     4141 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 
     4201 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 
     4261 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 
     4321 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 
     4381 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 
     4441 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 
     4501 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 
     4561 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 
     4621 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 
     4681 agcggccgcg tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 
     4741 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 
     4801 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 
     4861 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 
     4921 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 
     4981 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 
     5041 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc 
     5101 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 
     5161 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 
     5221 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc 
     5281 ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc 
     5341 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac 
     5401 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 
     5461 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 
     5521 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 
     5581 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 
     5641 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg 
     5701 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 
     5761 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc 
     5821 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 
     5881 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 
     5941 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt 
     6001 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 
     6061 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 
     6121 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac 
     6181 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 
     6241 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 
     6301 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 
     6361 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 
     6421 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 
     6481 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 
     6541 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 
     6601 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc 
     6661 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 
     6721 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 
     6781 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 
     6841 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 
     6901 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 
     6961 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 
     7021 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 
     7081 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 
     7141 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 
     7201 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 
     7261 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 
     7321 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 
     7381 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 
     7441 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 
     7501 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 
     7561 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 
     7621 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 
     7681 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 
     7741 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 
     7801 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 
     7861 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 
     7921 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 
     7981 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 
     8041 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 
     8101 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 
     8161 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 
     8221 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 
     8281 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 
     8341 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 
     8401 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 
     8461 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 
     8521 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca 
     8581 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 
     8641 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 
     8701 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 
     8761 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact 
     8821 aaagggaaca aaagctggag ctgc 
//
