LOCUS       pLV-EF1a-GFP-IRES-Neo     9669 bp    DNA     circular     22-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7590..8450
                     /label=Amp(R)
     promoter        8651..9191
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6195..6429
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7004..7459)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6501..6631
                     /label=SV40 pA
     misc_feature    6632..6845
                     /label=SV40 ori
     misc_feature    5516..6112
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             4712..5506
                     /label=Neo
     RBS             4116..4700
                     /label=IRES
     CDS             3375..4094
                     /label=GFP
BASE COUNT     2315 a      2471 c      2588 g      2295 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgatatcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 
     3421 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 
     3481 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 
     3541 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc 
     3601 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 
     3661 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 
     3721 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 
     3781 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 
     3841 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 
     3901 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 
     3961 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 
     4021 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 
     4081 agctgtacaa gtaaccaaaa aaatggcggc cgctagcccc tctccctccc ccccccctaa 
     4141 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 
     4201 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 
     4261 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 
     4321 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 
     4381 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 
     4441 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 
     4501 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 
     4561 accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc 
     4621 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 
     4681 acgatgataa tatggccaca ctagagccac catgattgaa caagatggat tgcacgcagg 
     4741 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 
     4801 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 
     4861 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct 
     4921 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 
     4981 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 
     5041 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 
     5101 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 
     5161 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 
     5221 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 
     5281 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 
     5341 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 
     5401 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 
     5461 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg ccgcgtcgac 
     5521 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 
     5581 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 
     5641 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 
     5701 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 
     5761 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 
     5821 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 
     5881 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 
     5941 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 
     6001 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt 
     6061 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga 
     6121 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 
     6181 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg 
     6241 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 
     6301 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 
     6361 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 
     6421 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 
     6481 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca 
     6541 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 
     6601 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc 
     6661 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 
     6721 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 
     6781 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg 
     6841 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 
     6901 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 
     6961 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc 
     7021 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 
     7081 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 
     7141 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 
     7201 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 
     7261 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 
     7321 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 
     7381 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 
     7441 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 
     7501 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 
     7561 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 
     7621 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 
     7681 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 
     7741 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 
     7801 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 
     7861 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 
     7921 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 
     7981 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 
     8041 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 
     8101 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 
     8161 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 
     8221 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 
     8281 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 
     8341 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 
     8401 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 
     8461 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 
     8521 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 
     8581 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 
     8641 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 
     8701 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 
     8761 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 
     8821 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 
     8881 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 
     8941 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 
     9001 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 
     9061 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 
     9121 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 
     9181 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 
     9241 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 
     9301 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 
     9361 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 
     9421 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 
     9481 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 
     9541 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 
     9601 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc 
     9661 tggagctgc 
//
