LOCUS       pLV-EF1a-GFP-IRES-Hyg     9912 bp    DNA     circular     22-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7833..8693
                     /label=Amp(R)
     promoter        8894..9434
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6438..6672
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7247..7702)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6744..6874
                     /label=SV40 pA
     misc_feature    6875..7088
                     /label=SV40 ori
     misc_feature    5759..6355
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             4724..5749
                     /label=Hyg
     RBS             4116..4700
                     /label=IRES
     CDS             3375..4094
                     /label=GFP
BASE COUNT     2384 a      2533 c      2656 g      2339 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgatatcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 
     3421 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 
     3481 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 
     3541 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc 
     3601 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 
     3661 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 
     3721 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 
     3781 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 
     3841 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 
     3901 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 
     3961 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 
     4021 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 
     4081 agctgtacaa gtaaccaaaa aaatggcggc cgctagcccc tctccctccc ccccccctaa 
     4141 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 
     4201 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 
     4261 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 
     4321 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 
     4381 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 
     4441 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 
     4501 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 
     4561 accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc 
     4621 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 
     4681 acgatgataa tatggccaca ctagagatcc accggtcgcc accatgaaaa agcctgaact 
     4741 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat 
     4801 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata 
     4861 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca 
     4921 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag 
     4981 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac 
     5041 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga 
     5101 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac 
     5161 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat 
     5221 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga 
     5281 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac 
     5341 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca 
     5401 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac 
     5461 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat 
     5521 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc 
     5581 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg 
     5641 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc 
     5701 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagg cggccgcgtc 
     5761 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 
     5821 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 
     5881 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 
     5941 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 
     6001 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 
     6061 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 
     6121 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg 
     6181 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 
     6241 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 
     6301 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt 
     6361 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 
     6421 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt 
     6481 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 
     6541 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 
     6601 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 
     6661 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 
     6721 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 
     6781 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 
     6841 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc 
     6901 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 
     6961 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 
     7021 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac 
     7081 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 
     7141 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 
     7201 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc 
     7261 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 
     7321 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 
     7381 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 
     7441 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 
     7501 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 
     7561 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 
     7621 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 
     7681 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta 
     7741 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 
     7801 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 
     7861 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 
     7921 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 
     7981 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 
     8041 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 
     8101 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 
     8161 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 
     8221 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 
     8281 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 
     8341 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 
     8401 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 
     8461 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 
     8521 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 
     8581 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 
     8641 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 
     8701 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 
     8761 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 
     8821 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 
     8881 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 
     8941 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 
     9001 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 
     9061 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 
     9121 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 
     9181 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 
     9241 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 
     9301 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 
     9361 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 
     9421 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 
     9481 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 
     9541 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 
     9601 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 
     9661 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 
     9721 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 
     9781 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 
     9841 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa 
     9901 agctggagct gc 
//
