LOCUS       pLV-EF1a-GFP-IRES-Bsd        9285 bp    DNA     circular     22-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7206..8066
                     /label=Amp(R)
     promoter        8267..8807
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5811..6045
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6620..7075)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6117..6247
                     /label=SV40 pA
     misc_feature    6248..6461
                     /label=SV40 ori
     misc_feature    5132..5728
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             4724..5122
                     /label=Bsd
     RBS             4116..4700
                     /label=IRES
     CDS             3375..4094
                     /label=GFP
BASE COUNT     2262 a      2356 c      2456 g      2211 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttggat 
     3361 ccgatatcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 
     3421 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 
     3481 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 
     3541 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc 
     3601 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 
     3661 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 
     3721 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 
     3781 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 
     3841 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 
     3901 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 
     3961 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 
     4021 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 
     4081 agctgtacaa gtaaccaaaa aaatggcggc cgctagcccc tctccctccc ccccccctaa 
     4141 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 
     4201 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 
     4261 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 
     4321 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 
     4381 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 
     4441 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 
     4501 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 
     4561 accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc 
     4621 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 
     4681 acgatgataa tatggccaca ctagagatcc accggtcgcc accatggcca agcctttgtc 
     4741 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc 
     4801 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt 
     4861 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc 
     4921 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat 
     4981 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc 
     5041 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc 
     5101 tggttatgtg tgggagggct aagcggccgc gtcgacaatc aacctctgga ttacaaaatt 
     5161 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 
     5221 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 
     5281 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 
     5341 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 
     5401 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 
     5461 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 
     5521 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg 
     5581 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 
     5641 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 
     5701 atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga 
     5761 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc 
     5821 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag 
     5881 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 
     5941 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 
     6001 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 
     6061 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact 
     6121 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     6181 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 
     6241 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 
     6301 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 
     6361 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac 
     6421 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa 
     6481 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct 
     6541 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 
     6601 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 
     6661 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 
     6721 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 
     6781 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 
     6841 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 
     6901 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 
     6961 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 
     7021 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg 
     7081 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
     7141 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 
     7201 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 
     7261 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 
     7321 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 
     7381 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 
     7441 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 
     7501 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 
     7561 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 
     7621 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 
     7681 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 
     7741 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 
     7801 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 
     7861 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 
     7921 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 
     7981 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 
     8041 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 
     8101 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 
     8161 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 
     8221 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 
     8281 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 
     8341 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 
     8401 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 
     8461 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 
     8521 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 
     8581 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 
     8641 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 
     8701 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 
     8761 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 
     8821 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 
     8881 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 
     8941 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 
     9001 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 
     9061 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 
     9121 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 
     9181 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 
     9241 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgc 
//
