LOCUS       pLV-EF1a-GFP-Bsd        8643 bp    DNA     circular     22-JAN-2013
COMMENT     
COMMENT     This file is created by Biosettia Inc
            http://www.biosettia.com/

FEATURES             Location/Qualifiers
     CDS             6564..7424
                     /label=Amp(R)
     promoter        7625..8165
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5169..5403
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(5978..6433)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     misc_feature    5606..5819
                     /label=SV40 ori
     misc_feature    4490..5086
                     /label=WPRE
     promoter        1933..3322
                     /label=human EF1a promoter
     intron          2361..3303
                     /label=intron
     CDS             3347..4063
                     /label=GFP
     CDS             4082..4480
                     /label=Bsd
     misc_feature    1807..1929
                     /label=cPPT
BASE COUNT     2105 a      2186 c      2293 g      2059 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3301 caggtgtcgt gaaaactacc cctctagaga tccaccggtc gccaccatgg tgagcaaggg 
     3361 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 
     3421 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 
     3481 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 
     3541 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 
     3601 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 
     3661 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 
     3721 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 
     3781 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 
     3841 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 
     3901 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 
     3961 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 
     4021 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aaggacccac cggtcgccac 
     4081 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat 
     4141 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg 
     4201 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt 
     4261 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg 
     4321 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct 
     4381 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat 
     4441 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcggccgcgt cgacaatcaa 
     4501 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 
     4561 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 
     4621 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 
     4681 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 
     4741 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 
     4801 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 
     4861 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 
     4921 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 
     4981 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 
     5041 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcgg 
     5101 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 
     5161 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta 
     5221 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 
     5281 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 
     5341 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 
     5401 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 
     5461 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 
     5521 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 
     5581 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc 
     5641 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 
     5701 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 
     5761 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca 
     5821 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 
     5881 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 
     5941 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta 
     6001 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 
     6061 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 
     6121 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 
     6181 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 
     6241 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 
     6301 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 
     6361 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 
     6421 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 
     6481 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 
     6541 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 
     6601 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 
     6661 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 
     6721 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 
     6781 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 
     6841 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 
     6901 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 
     6961 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 
     7021 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 
     7081 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 
     7141 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 
     7201 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 
     7261 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 
     7321 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 
     7381 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 
     7441 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 
     7501 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 
     7561 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 
     7621 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 
     7681 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 
     7741 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 
     7801 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 
     7861 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 
     7921 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 
     7981 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 
     8041 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 
     8101 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 
     8161 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 
     8221 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 
     8281 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 
     8341 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 
     8401 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 
     8461 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 
     8521 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 
     8581 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc 
     8641 tgc 
//
